Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 104% activity at 0.1 mM | Phaseolus vulgaris | |
additional information | not affected by citrate | Phaseolus vulgaris | |
NaF | 114% activity at 0.01 mM | Phaseolus vulgaris | |
Tartrate | 110% activity at 0.1 mM | Phaseolus vulgaris |
Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Phaseolus vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 94% residual activity at 0.1 mM | Phaseolus vulgaris | |
Mo6+ | 13% residual activity at 0.1 mM | Phaseolus vulgaris | |
VO43- | 5% residual activity at 0.01 mM | Phaseolus vulgaris | |
Zn2+ | 70% residual activity at 0.1 mM | Phaseolus vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
p-nitrophenyl phosphate | wild type enzyme | Phaseolus vulgaris | |
0.61 | - |
p-nitrophenyl phosphate | recombinant enzyme | Phaseolus vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | 108% activity at 0.01 mM | Phaseolus vulgaris | |
Mn2+ | 106% activity at 0.01 mM | Phaseolus vulgaris | |
Zn2+ | 101% activity at 0.01 mM | Phaseolus vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
1 * 56000 + 1 * 45000, gel filtration, recombinant enzyme | Phaseolus vulgaris |
56000 | - |
1 * 56000 + 1 * 45000, gel filtration, recombinant enzyme | Phaseolus vulgaris |
110000 | - |
gel filtration, recombinant enzyme | Phaseolus vulgaris |
120000 | - |
SDS-PAGE, recombinant enzyme | Phaseolus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | - |
variety kidney bean, cultivar Ohfuku | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Phaseolus vulgaris |
Purification (Comment) | Organism |
---|---|
ProBond resin nickel affinity column chromatography | Phaseolus vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.3 | - |
after 1fold purification, using p-nitrophenyl phosphate as a substrate | Phaseolus vulgaris |
32.2 | - |
after 25fold purification, using p-nitrophenyl phosphate as a substrate | Phaseolus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phosphoglycerate + H2O | 32% activity compared to p-nitrophenyl phosphate | Phaseolus vulgaris | glycerol + phosphate | - |
? | |
3-phosphoglycerate + H2O | 34% activity compared to p-nitrophenyl phosphate | Phaseolus vulgaris | glycerol + phosphate | - |
? | |
ADP + H2O | 54% activity compared to p-nitrophenyl phosphate | Phaseolus vulgaris | AMP + phosphate | - |
? | |
alpha-naphthylphosphate + H2O | 32% activity compared to p-nitrophenyl phosphate | Phaseolus vulgaris | alpha-naphthol + phosphate | - |
? | |
AMP + H2O | 13% activity compared to p-nitrophenyl phosphate | Phaseolus vulgaris | adenosine + phosphate | - |
? | |
ATP + H2O | 40% activity compared to p-nitrophenyl phosphate | Phaseolus vulgaris | ADP + phosphate | - |
? | |
beta-naphthylphosphate + H2O | 59% activity compared to p-nitrophenyl phosphate | Phaseolus vulgaris | beta-naphthol + phosphate | - |
? | |
D-glucose 1-phosphate + H2O | 58% activity compared to p-nitrophenyl phosphate | Phaseolus vulgaris | D-glucose + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | 46% activity compared to p-nitrophenyl phosphate | Phaseolus vulgaris | D-glucose + phosphate | - |
? | |
diphosphate + H2O | 65% activity compared to phosphoenolpyruvate | Phaseolus vulgaris | 2 phosphate | - |
? | |
GTP + H2O | 16% activity compared to phosphoenolpyruvate | Phaseolus vulgaris | GDP + phosphate | - |
? | |
additional information | the enzyme shows chloroperoxidase activity after the addition of orthovanadate, no activity with bis-p-nitrophenyl phosphate | Phaseolus vulgaris | ? | - |
? | |
p-nitrophenyl phosphate + H2O | 100% activity | Phaseolus vulgaris | p-nitrophenol + phosphate | - |
? | |
phosphoenolpyruvate + H2O | 67% activity compared to p-nitrophenyl phosphate | Phaseolus vulgaris | pyruvate + phosphate | - |
? | |
phosphothreonine + H2O | 42% activity compared to p-nitrophenyl phosphate | Phaseolus vulgaris | threonine + phosphate | - |
? | |
phosphotyrosine + H2O | 63% activity compared to p-nitrophenyl phosphate | Phaseolus vulgaris | tyrosine + phosphate | - |
? | |
phytate + H2O | 16% activity compared to p-nitrophenyl phosphate | Phaseolus vulgaris | ? + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 56000 + 1 * 45000, gel filtration, recombinant enzyme | Phaseolus vulgaris |
Synonyms | Comment | Organism |
---|---|---|
KhACP | shows also chloroperoxidase activity | Phaseolus vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Phaseolus vulgaris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | - |
Phaseolus vulgaris |