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Literature summary for 3.1.3.2 extracted from

  • Kaur, P.; Lingner, A.; Singh, B.; Boeer, E.; Polajeva, J.; Steinborn, G.; Bode, R.; Gellissen, G.; Satyanarayana, T.; Kunze, G.
    APHO1 from the yeast Arxula adeninivorans encodes an acid phosphatase of broad substrate specificity (2007), Antonie van Leeuwenhoek, 91, 45-55.
    View publication on PubMed
Search for more literature for 3.1.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Arxula adeninivorans strain G1211 Blastobotrys adeninivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 phytic acid recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
0.5
-
 3-indoxyl phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
0.7
-
 pyridoxal 5'-phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
1.4
-
 ADP recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
1.4
-
 AMP recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
1.7
-
 D-glucose 6-phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
1.8
-
 Sodium diphosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
2.5
-
 p-nitrophenyl phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
2.5
-
 1-naphthyl phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
2.5
-
 Glycerol 2-phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52400
-
 6 * 52400, calculated from amino acid sequence Blastobotrys adeninivorans
58330
-
 6 * 58330, gel filtration Blastobotrys adeninivorans
314400
-
 calculated from amino acid sequence Blastobotrys adeninivorans
350000
-
 gel filtration Blastobotrys adeninivorans

Organism

Organism UniProt Comment Textmining
Blastobotrys adeninivorans
-
-
-
Blastobotrys adeninivorans LS3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
hydroxylapatite column chromatography and Superdex 200 gel filtration Blastobotrys adeninivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl phosphate + H2O
-
 Blastobotrys adeninivorans 1-naphthol + phosphate
-
 ?
1-naphthyl phosphate + H2O
-
 Blastobotrys adeninivorans LS3 1-naphthol + phosphate
-
 ?
3-indoxyl phosphate + H2O
-
 Blastobotrys adeninivorans 3-indoxol + phosphate
-
 ?
ADP + H2O
-
 Blastobotrys adeninivorans AMP + phosphate
-
 ?
D-glucose 6-phosphate + H2O
-
 Blastobotrys adeninivorans D-glucose + phosphate
-
 ?
D-glucose 6-phosphate + H2O
-
 Blastobotrys adeninivorans LS3 D-glucose + phosphate
-
 ?
glycerol 2-phosphate + H2O
-
 Blastobotrys adeninivorans glycerol + phosphate
-
 ?
glycerol 2-phosphate + H2O
-
 Blastobotrys adeninivorans LS3 glycerol + phosphate
-
 ?
additional information no activity with cAMP Blastobotrys adeninivorans ?
-
 ?
additional information no activity with cAMP Blastobotrys adeninivorans LS3 ?
-
 ?
p-nitrophenyl phosphate + H2O
-
 Blastobotrys adeninivorans p-nitrophenol + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Blastobotrys adeninivorans LS3 p-nitrophenol + phosphate
-
 ?
phytic acid + H2O
-
 Blastobotrys adeninivorans ? + phosphate
-
 ?
pyridoxal 5'-phosphate + H2O
-
 Blastobotrys adeninivorans pyridoxal + phosphate
-
 ?
sodium diphosphate + H2O
-
 Blastobotrys adeninivorans sodium phosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 52400, calculated from amino acid sequence Blastobotrys adeninivorans
hexamer 6 * 58330, gel filtration Blastobotrys adeninivorans

Synonyms

Synonyms Comment Organism
Apho1p
-
 Blastobotrys adeninivorans
orthophosphoric-monoester phosphohydrolase
-
 Blastobotrys adeninivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Blastobotrys adeninivorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 60
-
 Blastobotrys adeninivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
845
-
 AMP recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
915
-
 phytic acid recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
1056
-
 3-indoxyl phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
1831
-
 Glycerol 2-phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
1901
-
 pyridoxal 5'-phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
2042
-
 Sodium diphosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
2462
-
 D-glucose 6-phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
2676
-
 ADP recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
5282
-
 1-naphthyl phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
40850
-
 p-nitrophenyl phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
 Blastobotrys adeninivorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.2 5.3
-
 Blastobotrys adeninivorans

pI Value

Organism Comment pI Value Maximum pI Value
Blastobotrys adeninivorans isoelectric focusing
-
 5.13