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Literature summary for 3.1.3.2 extracted from

  • Gonnety, J.T.; Niamke, S.; Faulet, B.M.; Jean-Parfait, E.; Kouadio, N.; Kouame, L.P.
    Purification and characterization of three low-molecular-weight acid phosphatases from peanut (Arachis hypogaea) seedlings (2006), Afr. J. Biotechnol., 5, 035-044.
No PubMed abstract available
Search for more literature for 3.1.3.2

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 136.55% activity at 0.1% (w/v) Arachis hypogaea
additional information not affected by L-cysteine, tetradecyl-trimethyl-ammoniumbromide 0.1% (w/v) and hexadecyl-trimethyl-ammoniumbromide 0.1% (w/v) Arachis hypogaea
Nonidet P 40 107.8% activity at 1% (w/v) Arachis hypogaea
Nonidet P 40 146.5% activity at 1% (w/v) Arachis hypogaea
Nonidet P 40 166.7% activity at 1% (w/v) Arachis hypogaea
polyoxyethylene-9-lauryl ether 120.4% activity at 1% (w/v) Arachis hypogaea
polyoxyethylene-9-lauryl ether 125% activity at 1% (w/v) Arachis hypogaea
polyoxyethylene-9-lauryl ether 147.4% activity at 1% (w/v) Arachis hypogaea
taurocholic acid 114% activity at 1% (w/v) Arachis hypogaea
taurocholic acid 136.3% activity at 1% (w/v) Arachis hypogaea
taurocholic acid 168.3% activity at 1% (w/v) Arachis hypogaea
Triton X-100 124.5% activity at 1% (w/v) Arachis hypogaea
Triton X-100 132.5% activity at 1% (w/v) Arachis hypogaea
Triton X-100 169.6% activity at 1% (w/v) Arachis hypogaea

General Stability

General Stability Organism
stable in the presence of Na+ and K+ Arachis hypogaea

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithio-2,2' dinitro-dibenzoate 13.26% residual activity at 0.1% (w/v); 39.83% residual activity at 0.1% (w/v); 9.1% residual activity at 0.1% (w/v) Arachis hypogaea
Ba2+ 30.3% residual activity at 1 mM; 50.9% residual activity at 1 mM Arachis hypogaea
beta-mercaptoethanol 72.72% residual activity at 0.1% (w/v); 75.98% residual activity at 0.1% (w/v); 92.8% residual activity at 0.1% (w/v) Arachis hypogaea
Ca2+ 80.2% residual activity at 5 mM; 86.2% residual activity at 5 mM Arachis hypogaea
Cu2+ 25.7% residual activity at 5 mM; 30% residual activity at 5 mM; 64.8% residual activity at 1 mM Arachis hypogaea
dithiothreitol 64.34% activity at 0.1% (w/v); 98.7% residual activity at 0.1% (w/v) Arachis hypogaea
EDTA 40% residual activity at 5 mM; 85% residual activity at 5 mM; 92.2% residual activity at 5 mM Arachis hypogaea
Fe3+ 76% residual activity at 5 mM; 85% residual activity at 5 mM Arachis hypogaea
hexadecyl-trimethyl-ammoniumbromide 87.8% residual activity at 0.1% (w/v) Arachis hypogaea
K+ 92.6% residual activity at 1 mM; 93.4% residual activity at 1 mM Arachis hypogaea
L-cysteine 72.72% residual activity at 0.1% (w/v); 85.27% residual activity at 0.1% (w/v) Arachis hypogaea
Mg2+ 66.5% residual activity at 5 mM; 90.8% residual activity at 5 mM Arachis hypogaea
p-chloromercuribenzoate 62.99% residual activity at 0.1% (w/v); 69.39% residual activity at 0.1% (w/v); 72.81% residual activity at 0.1% (w/v) Arachis hypogaea
SDS 10% residual activity at 0.1% (w/v); 22% residual activity at 0.1% (w/v); 3% residual activity at 0.1% (w/v) Arachis hypogaea
Sr2+ 45.7% residual activity at 5 mM Arachis hypogaea
tetradecyl-trimethyl-ammoniumbromide 50.6% residual activity at 0.1% (w/v) Arachis hypogaea
Zn2+ 15% residual activity at 5 mM; 19% residual activity at 5 mM; 3.1% residual activity at 5 mM Arachis hypogaea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
 Phenylphosphate at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
0.49
-
 p-nitrophenyl phosphate at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
0.76
-
 p-nitrophenyl phosphate at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
0.79
-
 p-nitrophenyl phosphate at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
1.16
-
 ATP at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
1.31
-
 Phenylphosphate at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
1.48
-
 ADP at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
1.5 2 Phenylphosphate at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
1.77
-
 ATP at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
2.88
-
 ATP at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
4.63
-
 ADP at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea
5.71
-
 ADP at 37°C in 100 mM sodium acetate buffer (pH 5.0) Arachis hypogaea

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 115% activity at 5 mM Arachis hypogaea
Ba2+ 223.3% activity at 5 mM Arachis hypogaea
Ca2+ 244% activity at 1 mM Arachis hypogaea
Cu2+ 190% activity at 5 mM Arachis hypogaea
Fe3+ 186% activity at 1 mM Arachis hypogaea
K+ 124.9% activity at 1 mM Arachis hypogaea
K+ 127.3% activity at 5 mM Arachis hypogaea
Mg2+ 143.6% activity at 5 mM Arachis hypogaea
Mg2+ 197% activity at 1 mM Arachis hypogaea
Na+ 118% activity at 5 mM Arachis hypogaea
Na+ 125% activity at 5 mM Arachis hypogaea
Sr2+ 131% activity at 5 mM Arachis hypogaea
Sr2+ 193% activity at 5 mM Arachis hypogaea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
 1 * 14000 + 1 * 12000, isozyme PI, gel filtration Arachis hypogaea
14000
-
 1 * 14000 + 1 * 12000, isozyme PI, gel filtration Arachis hypogaea
22400
-
 isozyme PIIa, SDS-PAGE Arachis hypogaea
24000
-
 isozyme PIIb, SDS-PAGE Arachis hypogaea
25300
-
 isozyme PI, SDS-PAGE Arachis hypogaea
26000
-
 isozyme PI, gel filtration Arachis hypogaea

Organic Solvent Stability

Organic Solvent Comment Organism
Nonidet P 40 stable in the presence of non-ionic detergents such as Nonidet P 40 Arachis hypogaea
Triton X-100 stable in the presence of non-ionic detergents such as Triton X-100 Arachis hypogaea

Organism

Organism UniProt Comment Textmining
Arachis hypogaea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose CL-6B chromatography, CM-Sepharose CL-6B chromatography, Sephacryl S-200 HR gel filtration, and Phenyl-Sepharose HP column chromatography Arachis hypogaea

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Arachis hypogaea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.62
-
 crude cell extract Arachis hypogaea
30.5
-
 isozyme PIIa, after 49.5fold purification Arachis hypogaea
36
-
 isozyme PIIb, after 58.2fold purification Arachis hypogaea
54.7
-
 isozyme PI, after 92fold purification Arachis hypogaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine-2',3'-cyclomonophosphate + H2O 63% activity compared to p-nitrophenyl phosphate Arachis hypogaea ?
-
 ?
adenosine-2',3'-cyclomonophosphate + H2O 9% activity compared to p-nitrophenyl phosphate Arachis hypogaea ?
-
 ?
ADP + H2O 100.2% activity compared to p-nitrophenyl phosphate Arachis hypogaea AMP + phosphate
-
 ?
ADP + H2O 103.4% activity compared to p-nitrophenyl phosphate Arachis hypogaea AMP + phosphate
-
 ?
ADP + H2O 81.5% activity compared to p-nitrophenyl phosphate Arachis hypogaea AMP + phosphate
-
 ?
AMP + H2O 25.2% activity compared to p-nitrophenyl phosphate Arachis hypogaea adenosine + phosphate
-
 ?
AMP + H2O 25.5% activity compared to p-nitrophenyl phosphate Arachis hypogaea adenosine + phosphate
-
 ?
AMP + H2O 51.5% activity compared to p-nitrophenyl phosphate Arachis hypogaea adenosine + phosphate
-
 ?
ATP + H2O 116.7% activity compared to p-nitrophenyl phosphate Arachis hypogaea ADP + phosphate
-
 ?
ATP + H2O 76.9% activity compared to p-nitrophenyl phosphate Arachis hypogaea ADP + phosphate
-
 ?
ATP + H2O 80.7% activity compared to p-nitrophenyl phosphate Arachis hypogaea ADP + phosphate
-
 ?
D-galactose 1-phosphate + H2O 44.7% activity compared to p-nitrophenyl phosphate Arachis hypogaea D-galactose + phosphate
-
 ?
D-galactose 1-phosphate + H2O 8.8% activity compared to p-nitrophenyl phosphate Arachis hypogaea D-galactose + phosphate
-
 ?
D-glucose 1-phosphate + H2O 21.4% activity compared to p-nitrophenyl phosphate Arachis hypogaea D-glucose + phosphate
-
 ?
D-glucose 6-phosphate + H2O 30.4% activity compared to p-nitrophenyl phosphate Arachis hypogaea D-glucose + phosphate
-
 ?
diphosphate + H2O 56.1% activity compared to p-nitrophenyl phosphate Arachis hypogaea 2 phosphate
-
 ?
diphosphate + H2O 66.35% activity compared to p-nitrophenyl phosphate Arachis hypogaea 2 phosphate
-
 ?
additional information no activity with adenosine-2',3'-cyclomonophosphate, D-glucose 1-phosphate, and D-glucose 6-phosphate Arachis hypogaea ?
-
 ?
additional information no activity with D-glucose 1-phosphate, D-glucose 6-phosphate, and sodium phytate Arachis hypogaea ?
-
 ?
additional information no activity with uridyl-5'-phosphogalactose, D-galactose 1-phosphate, and sodium phytate Arachis hypogaea ?
-
 ?
NAD+ + H2O 15.4% activity compared to p-nitrophenyl phosphate Arachis hypogaea ?
-
 ?
NAD+ + H2O 16.5% activity compared to p-nitrophenyl phosphate Arachis hypogaea ?
-
 ?
NAD+ + H2O 26.6% activity compared to p-nitrophenyl phosphate Arachis hypogaea ?
-
 ?
NADP+ + H2O 28% activity compared to p-nitrophenyl phosphate Arachis hypogaea NAD+ + phosphate
-
 ?
NADP+ + H2O 36% activity compared to p-nitrophenyl phosphate Arachis hypogaea NAD+ + phosphate
-
 ?
NADP+ + H2O 61.7% activity compared to p-nitrophenyl phosphate Arachis hypogaea NAD+ + phosphate
-
 ?
p-nitrophenyl phosphate + H2O 100% activity Arachis hypogaea p-nitrophenol + phosphate
-
 ?
phenylphosphate + H2O 85.2% activity compared to p-nitrophenyl phosphate Arachis hypogaea phenol + phosphate
-
 ?
phenylphosphate + H2O 89% activity compared to p-nitrophenyl phosphate Arachis hypogaea phenol + phosphate
-
 ?
phenylphosphate + H2O 93.1% activity compared to p-nitrophenyl phosphate Arachis hypogaea phenol + phosphate
-
 ?
phytate + H2O 20.1% activity compared to p-nitrophenyl phosphate Arachis hypogaea ?
-
 ?
sodium diphosphate + H2O 87% activity compared to p-nitrophenyl phosphate Arachis hypogaea sodium phosphate + phosphate
-
 ?
uridyl-5'-phosphogalactose + H2O 21% activity compared to p-nitrophenyl phosphate Arachis hypogaea uridyl-5'-galactose + phosphate
-
 ?
uridyl-5'-phosphogalactose + H2O 25.5% activity compared to p-nitrophenyl phosphate Arachis hypogaea uridyl-5'-galactose + phosphate
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 14000 + 1 * 12000, isozyme PI, gel filtration Arachis hypogaea
monomer 1 * 22400, isozyme PIIa, SDS-PAGE Arachis hypogaea
monomer 1 * 24000, isozyme PIIb, SDS-PAGE Arachis hypogaea

Synonyms

Synonyms Comment Organism
acid phosphatase PI isozyme Arachis hypogaea
acid phosphatase PIIa isozyme, exhibits phytasic activity Arachis hypogaea
acid phosphatase PIIb isozyme Arachis hypogaea
orthophosphoric-monoester phosphohydrolase
-
 Arachis hypogaea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Arachis hypogaea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 80 above 55°C, the activity declines rapidly as the temperature increases, but the enzyme is not completely inactivated even at 80°C Arachis hypogaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Arachis hypogaea

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.6 5.6 at 37°C Arachis hypogaea
5 5.6 at 37°C Arachis hypogaea