Crystallization (Comment) | Organism |
---|---|
purified enzyme, 22 mg/ml protein in 0.1 M acetate, pH 4.9, mixed with well solution containing 0.1 M citric acid, pH 3.5-4.0, 7.5% PEG 6000, 10% isopropanol, 50 mM phosphate, and 10% glycerol, 4°C, cryoprotection by increase of glycerol concentration to 20%, X-ray diffraction structure determination and analysis at 2.5 A resolution | Ipomoea batatas |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | competitive, moderate to weak bonding interactions to the enzyme | Ipomoea batatas |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | phosphate forms an unusual tripodal complex with the Fe(III)-Mn(II) center, structure, oxygen binding and bridging at the metal ion center | Ipomoea batatas | |
Mn2+ | strictly required, phosphate forms an unusual tripodal complex with the Fe(III)-Mn(II) center, structure, oxygen binding and bridging at the metal ion center | Ipomoea batatas | |
phosphate | forms an unusual tripodal complex with the Fe(III)-Mn(II) center, structure | Ipomoea batatas |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ipomoea batatas | Q9SE00 | cv. Golden | - |
Purification (Comment) | Organism |
---|---|
to over 95% purity | Ipomoea batatas |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a phosphate monoester + H2O = an alcohol + phosphate | active site structure analysis, substrate binding, H295 and E365 are involved in substrate orientation and stabilization of the transition state, oxygen binding and bridging at the metal ion center, overview, reaction mechanism | Ipomoea batatas |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the family of binuclear metalloenzymes | Ipomoea batatas |
PAP | - |
Ipomoea batatas |
purple acid phosphatase | - |
Ipomoea batatas |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | maximal turnover at neutral pH | Ipomoea batatas |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
forms a micro-oxo-bridge at pH 4.9, maximal turnover at neutral pH | Ipomoea batatas |
4.5 | - |
maximal catalytic efficiency | Ipomoea batatas |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8.5 | activity range | Ipomoea batatas |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
phosphate | pH 7.0 | Ipomoea batatas | |
0.196 | - |
phosphate | pH 3.5 | Ipomoea batatas | |
0.31 | - |
phosphate | pH 4.9 | Ipomoea batatas |