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Literature summary for 3.1.3.2 extracted from
- Partial purification and characterization of a non-specific acid phosphatase in leaves and root nodules of Phaseolus vulgaris (2004), Plant Physiol. Biochem., 42, 585-591.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | phosphate starvation induces the enzyme | Phaseolus vulgaris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | strong inhibition of leaf and root nodule isozymes | Phaseolus vulgaris |  |
| Co2+ | inhibition of leaf and root nodule isozymes | Phaseolus vulgaris | |
| Cu2+ | strong inhibition of leaf and root nodule isozymes | Phaseolus vulgaris | |
| Hg2+ | strong inhibition of leaf and root nodule isozymes | Phaseolus vulgaris | |
| K+ | slight inhibition of root nodule isozymes | Phaseolus vulgaris | |
| Li+ | inhibits isozyme alpha from leaf | Phaseolus vulgaris | |
| Mn2+ | inhibition of leaf and root nodule isozymes | Phaseolus vulgaris | |
| molybdate | strong inhibition of leaf and root nodule isozymes | Phaseolus vulgaris | |
| NH4+ | slight inhibition of root nodule isozymes | Phaseolus vulgaris | |
| Pb2+ | strong inhibition of leaf and root nodule isozymes | Phaseolus vulgaris | |
| Zn2+ | strong inhibition of leaf and root nodule isozymes | Phaseolus vulgaris | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Phaseolus vulgaris | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | leaf and root nodule isozymes are not affected by Mg2+ and Ca2+, the leaf enzyme is also not affected by NH4+, while the root nodule enzyme is not affected by Li+ | Phaseolus vulgaris |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
2 * 41000 + 1 * 34000, root nodule isozyme gamma enzyme, SDS-PAGE | Phaseolus vulgaris |
| 41000 | - |
2 * 41000 + 1 * 34000, root nodule isozyme gamma enzyme, SDS-PAGE | Phaseolus vulgaris |
| 49000 | - |
1 * 51000 + 1 * 72000, leaf isozyme alpha, SDS-PAGE, 1 * 49000 + 1 * 72000, root nodule isozyme beta, SDS-PAGE | Phaseolus vulgaris |
| 51000 | - |
1 * 51000 + 1 * 72000, leaf isozyme alpha, SDS-PAGE, 1 * 49000 + 1 * 72000, root nodule isozyme beta, SDS-PAGE | Phaseolus vulgaris |
| 72000 | - |
1 * 51000 + 1 * 72000, leaf isozyme alpha, SDS-PAGE, 1 * 49000 + 1 * 72000, root nodule isozyme beta, SDS-PAGE | Phaseolus vulgaris |
| 110000 | - |
isozyme beta of root nodule, gel filtration | Phaseolus vulgaris |
| 116000 | - |
leaf isozyme alpha, gel filtration | Phaseolus vulgaris |
| 123000 | - |
isozyme gamma of root nodule, gel filtration | Phaseolus vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
var. Contender, three isozymes alpha, beta, and gamma | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 120.7fold from root nodules and leaves by ammonium sulfate fractionation, DEAE ion exchange chromatography, ultrafiltration, and gel filtration | Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | single isozyme alpha | Phaseolus vulgaris | - |
| root nodule | two isozymes beta and gamma | Phaseolus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 106.3 | - |
purified enzyme | Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | high activity | Phaseolus vulgaris | 4-nitrophenol + phosphate | - |
? | |
| alpha-naphthyl phosphate + H2O | high activity | Phaseolus vulgaris | alpha-naphthol + phosphate | - |
? | |
| ATP + H2O | - |
Phaseolus vulgaris | ADP + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | - |
Phaseolus vulgaris | D-glucose + phosphate | - |
? | |
| FMN + H2O | - |
Phaseolus vulgaris | ? + phosphate | - |
? | |
| additional information | substrate specificity of leaf and root nodule enzymes, overview | Phaseolus vulgaris | ? | - |
? | |
| phosphoenolpyruvate + H2O | - |
Phaseolus vulgaris | pyruvate + phosphate | - |
? | |
| ribulose 1,5-bisphosphate + H2O | high activity | Phaseolus vulgaris | ? + phosphate | - |
? | |
| uridine 5'-phosphate + H2O | - |
Phaseolus vulgaris | uridine + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 51000 + 1 * 72000, leaf isozyme alpha, SDS-PAGE, 1 * 49000 + 1 * 72000, root nodule isozyme beta, SDS-PAGE | Phaseolus vulgaris |
| trimer | 2 * 41000 + 1 * 34000, root nodule isozyme gamma enzyme, SDS-PAGE | Phaseolus vulgaris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACP | - |
Phaseolus vulgaris |
| non-specific acid phosphatase | - |
Phaseolus vulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Phaseolus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
- |
Phaseolus vulgaris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | inactive below pH 4.0 and above pH 7.0 | Phaseolus vulgaris |
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