Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is induced by betaine, choline, and N,N-dimethylglycine as sole carbon and nitrogen sources | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphorylcholine | substrate inhibition | Pseudomonas aeruginosa | |
Phosphorylethanolamine | substrate inhibition | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Pseudomonas aeruginosa | |
0.2 | - |
phosphorylcholine | pH 5.0, 37°C | Pseudomonas aeruginosa | |
0.7 | - |
Phosphorylethanolamine | pH 5.0, 37°C | Pseudomonas aeruginosa | |
2 | - |
4-nitrophenyl phosphate | pH 5.0, 37°C | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Pseudomonas aeruginosa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially by ammonium sulfate fractionation and gel filtration | Pseudomonas aeruginosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the enzyme is induced by betaine, choline, and N,N-dimethylglycine as sole carbon and nitrogen sources | Pseudomonas aeruginosa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + phosphate | - |
? | |
additional information | the enzyme shows high substrate specificity, no activity with pyridoxal 5'-phosphate, glucose 6-phosphate, fructose 6-phosphate, fructose 1,6-diphosphate, AMP, ADP, and ATP | Pseudomonas aeruginosa | ? | - |
? | |
phosphorylcholine + H2O | preferred substrate compared to phosphorylethanolamine | Pseudomonas aeruginosa | choline + phosphate | - |
? | |
phosphorylethanolamine + H2O | - |
Pseudomonas aeruginosa | ethanolamine + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Pseudomonas aeruginosa | |
1 | - |
phosphorylcholine | pH 5.0, 37°C | Pseudomonas aeruginosa | |
3 | - |
Phosphorylethanolamine | pH 5.0, 37°C | Pseudomonas aeruginosa |