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Literature summary for 3.1.3.2 extracted from

  • Funhoff, E.G.; Wang, Y.; Andersson, G.; Averill, B.A.
    Substrate positioning by His92 is important in catalysis by purple acid phosphatase (2005), FEBS J., 272, 2968-2977.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
H92A site-directed mutagenesis, the mutation shifts the pH optimum, and eliminates the polar interactions of His92 with the active site and decreases the activity by 10fold at optimal pH Homo sapiens
H92N site-directed mutagenesis, the mutation shifts the pH optimum, and alters interactions of His92 with the active site and increases the activity by 3fold at optimal pH Homo sapiens
N91A site-directed mutagenesis Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of recombinant wild-type and mutant enzymes Homo sapiens
2 3 4-nitrophenyl phosphate pH 4.4, recombinant mutant H92N Homo sapiens
4.5
-
4-nitrophenyl phosphate pH 5.5, recombinant wild-type enzyme Homo sapiens
35
-
4-nitrophenyl phosphate pH 3.8, recombinant mutant H92A Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Asn91 ia a ligand to the divalent metal of the mixed-valent di-iron center Homo sapiens
Fe3+ the enzyme possesses a mixed-valent di-iron center Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
a phosphate monoester + H2O = an alcohol + phosphate Asn91 and His92 are active site residues which interact with the loop residue Asp146, this interaction and substrate positioning by His92 is important for catalysis, Asn91 ia a ligand to the divalent metal of the mixed-valent di-iron center Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Homo sapiens 4-nitrophenol + phosphate
-
?

Subunits

Subunits Comment Organism
monomer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
PAP
-
Homo sapiens
purple acid phosphatase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information pH dependency of kcat of recombinant wild-type and mutant enzymes Homo sapiens
21
-
4-nitrophenyl phosphate pH 3.8, recombinant mutant H92A Homo sapiens
240
-
4-nitrophenyl phosphate pH 5.5, recombinant wild-type enzyme Homo sapiens
760
-
4-nitrophenyl phosphate pH 4.4, recombinant mutant H92N Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pH profile and influence of kinetics for recombinant wild-type and mutant enzymes Homo sapiens
3.8
-
recombinant mutant H92A Homo sapiens
4.4
-
recombinant mutant H92N Homo sapiens
5.5
-
recombinant wild-type enzyme Homo sapiens