Protein Variants | Comment | Organism |
---|---|---|
H92A | site-directed mutagenesis, the mutation shifts the pH optimum, and eliminates the polar interactions of His92 with the active site and decreases the activity by 10fold at optimal pH | Homo sapiens |
H92N | site-directed mutagenesis, the mutation shifts the pH optimum, and alters interactions of His92 with the active site and increases the activity by 3fold at optimal pH | Homo sapiens |
N91A | site-directed mutagenesis | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of recombinant wild-type and mutant enzymes | Homo sapiens | |
2 | 3 | 4-nitrophenyl phosphate | pH 4.4, recombinant mutant H92N | Homo sapiens | |
4.5 | - |
4-nitrophenyl phosphate | pH 5.5, recombinant wild-type enzyme | Homo sapiens | |
35 | - |
4-nitrophenyl phosphate | pH 3.8, recombinant mutant H92A | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Asn91 ia a ligand to the divalent metal of the mixed-valent di-iron center | Homo sapiens | |
Fe3+ | the enzyme possesses a mixed-valent di-iron center | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a phosphate monoester + H2O = an alcohol + phosphate | Asn91 and His92 are active site residues which interact with the loop residue Asp146, this interaction and substrate positioning by His92 is important for catalysis, Asn91 ia a ligand to the divalent metal of the mixed-valent di-iron center | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PAP | - |
Homo sapiens |
purple acid phosphatase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pH dependency of kcat of recombinant wild-type and mutant enzymes | Homo sapiens | |
21 | - |
4-nitrophenyl phosphate | pH 3.8, recombinant mutant H92A | Homo sapiens | |
240 | - |
4-nitrophenyl phosphate | pH 5.5, recombinant wild-type enzyme | Homo sapiens | |
760 | - |
4-nitrophenyl phosphate | pH 4.4, recombinant mutant H92N | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profile and influence of kinetics for recombinant wild-type and mutant enzymes | Homo sapiens |
3.8 | - |
recombinant mutant H92A | Homo sapiens |
4.4 | - |
recombinant mutant H92N | Homo sapiens |
5.5 | - |
recombinant wild-type enzyme | Homo sapiens |