Literature summary for 3.1.3.2 extracted from

Guimaraes, L.H.S.; Terenzi, H.F.; Jorge, J.A.; Leone, F.A.; Polizeli, M.d.L.T.M.
Characterization and properties of acid phosphatases with phytase activity produced by Aspergillus caespitosus (2004), Biotechnol. Appl. Biochem., 40, 201-207.

Search for more literature for 3.1.3.2

Inhibitors

Inhibitors	Comment	Organism	Structure
KH2PO4	intra- and extracellular enzyme forms	Aspergillus caespitosus
additional information	intra- and extracellular enzyme forms are both resistant to tartrate	Aspergillus caespitosus
NaF	inhibition of the intracellular enzyme, only slight inhibition of the extracellular enzyme	Aspergillus caespitosus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.029	-	4-nitrophenyl phosphate	pH 5.6, 80°C, intracellular enzyme	Aspergillus caespitosus
0.082	-	4-nitrophenyl phosphate	pH 5.6, 80°C, extracellular enzyme	Aspergillus caespitosus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Aspergillus caespitosus	-	-
intracellular	-	Aspergillus caespitosus	5622	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
74000	-	2 * 84000, extracellular enzyme form, SDS-PAGE, 2 * 74000, intracellular enzyme form, SDS-PAGE	Aspergillus caespitosus
84000	-	2 * 84000, extracellular enzyme form, SDS-PAGE, 2 * 74000, intracellular enzyme form, SDS-PAGE	Aspergillus caespitosus
186000	-	extracellular enzyme form, gel filtration	Aspergillus caespitosus
190000	-	intracellular enzyme form, gel filtration	Aspergillus caespitosus

Organism

Organism	UniProt	Comment	Textmining
Aspergillus caespitosus	-	intra- and extracellular enzyme forms	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	both intra- and extracellular enzyme forms are glycoproteins showing 58.3% and 63.0% carbohydrate content, respectively	Aspergillus caespitosus

Purification (Commentary)

Purification (Comment)	Organism
separation of enzyme forms by ion exchange chromatography and concanavalin A affinity chromatography	Aspergillus caespitosus

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	optimal culture conditions for enzyme production are 40°C and pH 6.0	Aspergillus caespitosus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl phosphate + H2O	higher activity with extracellular enzyme	Aspergillus caespitosus	4-nitrophenol + phosphate	-	?
additional information	intra- and extracellular enzyme forms show also phytase activity	Aspergillus caespitosus	?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 84000, extracellular enzyme form, SDS-PAGE, 2 * 74000, intracellular enzyme form, SDS-PAGE	Aspergillus caespitosus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
80	-	intra- and extracellular enzyme forms	Aspergillus caespitosus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	stable for over 60 min, intra- and extracellular enzyme forms	Aspergillus caespitosus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	intra- and extracellular enzyme forms	Aspergillus caespitosus

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Release 2023.1 (January 2023)