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Literature summary for 3.1.3.2 extracted from

  • Janckila, A.J.; Parthasarathy, R.N.; Parthasarathy, L.K.; Seelan, R.S.; Yam, L.T.
    Stable expression of human tartrate-resistant acid phosphatase isoforms by CHO cells (2002), Clin. Chim. Acta, 326, 113-122.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
transfection of the human ACP 5 gene into CHO cells and cloning of a stable cell line (CHO/TRAP 8F5) that expresses high levels of TRACP activity both intracellularly and as a secreted product. Secreted recombinant TRACPs have all characteristics of natural serum TRACP 5a. The intracellular TRACPs have all the same characteristics of natiral serum TRACP 5b Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9
-
4-nitrophenyl phosphate recombinant extracellular enzyme, isoenzyme 5a Homo sapiens
13.9
-
4-nitrophenyl phosphate recombinant intracellular enzyme, isoenzyme 5b Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens isoenzyme 5a is an osteoclast product that reflects bone resorption rate and is frequently increased in disease of increased bone turnover. Isoform 5a is often increased in rheumatoid arthritis sera and may be a product of inflammatory macrophages ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the recombinant extracellular TRACP, isoenzyme 5a, exists of least as seven glycoproteins, differentially sialylated Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
serum two closely related isoforms: 5a and 5b Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate
-
Homo sapiens 4-nitrophenol + phosphate
-
?
additional information isoenzyme 5a is an osteoclast product that reflects bone resorption rate and is frequently increased in disease of increased bone turnover. Isoform 5a is often increased in rheumatoid arthritis sera and may be a product of inflammatory macrophages Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
TRACP
-
Homo sapiens
type-5 tartrate-resistant phosphatase
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
recombinant extracellular enzyme, isoenzyme 5a Homo sapiens
5.8
-
recombinant intracellular enzyme, isoenzyme 5b Homo sapiens