Cloned (Comment) | Organism |
---|---|
transfection of the human ACP 5 gene into CHO cells and cloning of a stable cell line (CHO/TRAP 8F5) that expresses high levels of TRACP activity both intracellularly and as a secreted product. Secreted recombinant TRACPs have all characteristics of natural serum TRACP 5a. The intracellular TRACPs have all the same characteristics of natiral serum TRACP 5b | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
4-nitrophenyl phosphate | recombinant extracellular enzyme, isoenzyme 5a | Homo sapiens | |
13.9 | - |
4-nitrophenyl phosphate | recombinant intracellular enzyme, isoenzyme 5b | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | isoenzyme 5a is an osteoclast product that reflects bone resorption rate and is frequently increased in disease of increased bone turnover. Isoform 5a is often increased in rheumatoid arthritis sera and may be a product of inflammatory macrophages | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the recombinant extracellular TRACP, isoenzyme 5a, exists of least as seven glycoproteins, differentially sialylated | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
serum | two closely related isoforms: 5a and 5b | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? | |
additional information | isoenzyme 5a is an osteoclast product that reflects bone resorption rate and is frequently increased in disease of increased bone turnover. Isoform 5a is often increased in rheumatoid arthritis sera and may be a product of inflammatory macrophages | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TRACP | - |
Homo sapiens |
type-5 tartrate-resistant phosphatase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
recombinant extracellular enzyme, isoenzyme 5a | Homo sapiens |
5.8 | - |
recombinant intracellular enzyme, isoenzyme 5b | Homo sapiens |