Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM CaCl2, 20% inhibition | Lens culinaris | |
F- | NaF; noncompetitive | Lens culinaris | |
phosphate | - |
Lens culinaris | |
Zn2+ | - |
Lens culinaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
p-nitrophenyl phosphate | - |
Lens culinaris | |
1.4 | - |
L-tyrosine-O-phosphate | - |
Lens culinaris | |
2 | - |
3'-AMP | - |
Lens culinaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
x * 24000, SDS-PAGE | Lens culinaris |
100000 | - |
gel filtration | Lens culinaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lens culinaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lens culinaris |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10 | - |
- |
Lens culinaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl phosphate + H2O | - |
Lens culinaris | 1-naphthol + phosphate | - |
? | |
2'-AMP + H2O | 7% of the activity with p-nitrophenyl phosphate | Lens culinaris | adenosine + phosphate | - |
? | |
2-glycerophosphate + H2O | 37% of the activity with p-nitrophenyl phosphate | Lens culinaris | glycerol + phosphate | - |
? | |
3'-AMP + H2O | 53% of the activity with p-nitrophenyl phosphate | Lens culinaris | adenosine + phosphate | - |
? | |
5'-AMP + H2O | 15% of the activity with p-nitrophenyl phosphate | Lens culinaris | adenosine + phosphate | - |
? | |
5'-GMP + H2O | 5% of the activity with p-nitrophenyl phosphate | Lens culinaris | guanosine + phosphate | - |
? | |
O-phospho-L-Tyr + H2O | 103% of the activity with p-nitrophenyl phosphate | Lens culinaris | Tyr + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Lens culinaris | p-nitrophenol + phosphate | - |
? | |
phosphoenolpyruvate + H2O | 20% of the activity with p-nitrophenyl phosphate | Lens culinaris | pyruvate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24000, SDS-PAGE | Lens culinaris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH 5.5, half-life for p-nitrophenyl phosphate hydrolyzing activity is 46 min, half-life of 3'-AMP hydrolyzing activity: 15 min | Lens culinaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Lens culinaris |