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Literature summary for 3.1.3.2 extracted from

  • Shimada, Y.; Shinmyo, A.; Enatsu, T.
    Purification and properties of one component of acid phosphatase produced by Aspergillus niger (1977), Biochim. Biophys. Acta, 480, 417-427.
    View publication on PubMed
Search for more literature for 3.1.3.2

Inhibitors

Inhibitors Comment Organism Structure
2-hydroyxy-5-nitrobenzyl bromide
-
 Aspergillus niger
arsenate
-
 Aspergillus niger
Borate
-
 Aspergillus niger
Bromosuccinimide
-
 Aspergillus niger
F-
-
 Aspergillus niger
Fe3+ 8 mM, 45% inhibition Aspergillus niger
molybdate
-
 Aspergillus niger
phosphate
-
 Aspergillus niger
Tartrate inhibition by L(+)-tartrate, D(-) tartrate has no effect Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
89000
-
 x * 89000, SDS-PAGE Aspergillus niger
310000
-
 gel filtration Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 29% carbohydrate consisting of glucosamine, mannose and galactose Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Aspergillus niger

Storage Stability

Storage Stability Organism
-15°C, pH 4.6, 0.05 M citrate buffer, stable for at least 3 months Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl phosphate + H2O 86% of the activity with p-nitrophenyl phosphate Aspergillus niger 1-naphthol + phosphate
-
 ?
2-glycerophosphate + H2O 72% of the activity with p-nitrophenyl phosphate Aspergillus niger glycerol + phosphate
-
 ?
5'-ADP + H2O 14% of the activity with p-nitrophenyl phosphate Aspergillus niger AMP + phosphate
-
 ?
5'-AMP + H2O 105% of the activity with p-nitrophenyl phosphate Aspergillus niger adenosine + phosphate
-
 ?
5'-CMP + H2O 97% of the activity with p-nitrophenyl phosphate Aspergillus niger cytidine + phosphate
-
 ?
5'-GMP + H2O 33% of the activity with p-nitrophenyl phosphate Aspergillus niger guanosine + phosphate
-
 ?
6-phosphogluconate + H2O 17% of the activity with p-nitrophenyl phosphate Aspergillus niger gluconate + phosphate
-
 ?
alpha-glycerophosphate + H2O 38% of the activity with p-nitrophenyl phosphate Aspergillus niger glycerol + phosphate
-
 ?
bis(p-nitrophenyl) phosphate + H2O 2% of the activity with p-nitrophenyl phosphate Aspergillus niger ?
-
 ?
D-fructose 1,6-diphosphate + H2O 4% of the activity with p-nitrophenyl phosphate Aspergillus niger ?
-
 ?
D-fructose 6-phosphate + H2O 32% of the activity with p-nitrophenyl phosphate Aspergillus niger D-fructose + phosphate
-
 ?
D-ribose 5-phosphate + H2O 36% of the activity with p-nitrophenyl phosphate Aspergillus niger D-ribose + phosphate
-
 ?
glucose 1-phosphate + H2O 45% of the activity with p-nitrophenyl phosphate Aspergillus niger glucose + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Aspergillus niger p-nitrophenol + phosphate
-
 ?
phosphopyruvate + H2O 40% of the activity with p-nitrophenyl phosphate Aspergillus niger pyruvate + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 89000, SDS-PAGE Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 hydrolysis of p-nitrophenyl phosphate Aspergillus niger