Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3), pET-22b expression vector | Shigella flexneri |
Crystallization (Comment) | Organism |
---|---|
recombinant protein, selenomethionine-labelled protein, hanging-drop vapour-diffusion method at 18°C, polyethylene glycol 3500 as a precipitant and zinc acetate as an additive, data-collection and processing statistics | Shigella flexneri |
General Stability | Organism |
---|---|
zinc coordination promotes the enzyme to a stable conformation | Shigella flexneri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
1 * 27000, SDS-PAGE | Shigella flexneri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shigella flexneri | Q83PX1 | 2a strain 301 | - |
Purification (Comment) | Organism |
---|---|
gel filtration, SDS-PAGE | Shigella flexneri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
catalytic mechanism, dimerization behaviour and influence of metal ions on enzyme activity, recombinant protein, crystallization indicates the presence of two molecules in the asymmetric unit, structure determination using the multiwavelength anomalous dispersion (MAD) method, indicates that enzyme bears an alpha-helical cap domain that differs from that of a previously reported orthologue | Shigella flexneri |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 27000, SDS-PAGE | Shigella flexneri |
Synonyms | Comment | Organism |
---|---|---|
SfPGPase | - |
Shigella flexneri |