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Literature summary for 3.1.3.18 extracted from

  • Liu, H.; Zhou, H.; Zhu, D.; Bi, R.
    Overexpression, purification, characterization and preliminary crystallographic study of phosphoglycolate phosphatase from Shigella flexneri 2a strain 301 (2009), Acta Crystallogr. Sect. F, F65, 29-33.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3), pET-22b expression vector Shigella flexneri

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant protein, selenomethionine-labelled protein, hanging-drop vapour-diffusion method at 18°C, polyethylene glycol 3500 as a precipitant and zinc acetate as an additive, data-collection and processing statistics Shigella flexneri

General Stability

General Stability Organism
zinc coordination promotes the enzyme to a stable conformation Shigella flexneri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
1 * 27000, SDS-PAGE Shigella flexneri

Organism

Organism UniProt Comment Textmining
Shigella flexneri Q83PX1 2a strain 301
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, SDS-PAGE Shigella flexneri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
catalytic mechanism, dimerization behaviour and influence of metal ions on enzyme activity, recombinant protein, crystallization indicates the presence of two molecules in the asymmetric unit, structure determination using the multiwavelength anomalous dispersion (MAD) method, indicates that enzyme bears an alpha-helical cap domain that differs from that of a previously reported orthologue Shigella flexneri

Subunits

Subunits Comment Organism
monomer 1 * 27000, SDS-PAGE Shigella flexneri

Synonyms

Synonyms Comment Organism
SfPGPase
-
Shigella flexneri