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Literature summary for 3.1.3.18 extracted from
- Phosphoglycolate phosphatase from human red blood cells (1981), Arch. Biochem. Biophys., 208, 602-609.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Homo sapiens |  |
| iodoacetate | - |
Homo sapiens | |
| Phosphoglycolate * Mg2+ complex | - |
Homo sapiens | |
| ZnCl2 | 20% inhibition at 0.2 mM, 50% inhibition at 0.5 mM | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km increases at higher pH, above 7.5 | Homo sapiens | |
| 1.5 | 2 | Phosphoglycolate | ethyl-phosphate, human | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens | |
| ZnCl2 | slight activation up to 0.1 mM | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, as an ammonium sulfate paste, few months, loss of activity | Homo sapiens |
| 4°C, 50 mM KCl, 2 mM MgCl2, 2.5 mg/ml bovine serum albumin, 3 weeks, 50% loss of activity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-Phosphoglycolate + H2O | - |
Homo sapiens | Glycolate + phosphate | - |
? | |
| Ethyl-phosphate + H2O | - |
Homo sapiens | Ethanol + phosphate | - |
? | |
| i-Propyl-phosphate + H2O | slowly | Homo sapiens | i-Propanol + phosphate | - |
? | |
| n-Propyl-phosphate + H2O | weak | Homo sapiens | n-Propanol + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | - |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9.5 | - |
Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
ZnCl2 | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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