Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. Expression of the enzyme in the pET3a vector results in the expression of the enzyme as an insoluble aggregate. The vector with the trp-lac hybrid promotor the enzyme is expressed as a soluble protein at levels of 3-4% of the soluble Escherichia coli protein | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
microcystin-LR | - |
Oryctolagus cuniculus | |
okadaic acid | - |
Oryctolagus cuniculus | |
protein | modulator protein, inhibitor 2 | Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | skeletal | Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphorylase a + H2O | - |
Oryctolagus cuniculus | phosphorylase b + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Oryctolagus cuniculus |
monomer | - |
Oryctolagus cuniculus |