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Literature summary for 3.1.3.16 extracted from

  • Ulrych, A.; Holeckova, N.; Goldova, J.; Doubravova, L.; Benada, O.; Kofronova, O.; Halada, P.; Branny, P.
    Characterization of pneumococcal Ser/Thr protein phosphatase phpP mutant and identification of a novel PhpP substrate, putative RNA binding protein Jag (2016), BMC Microbiol., 16, 247 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene phpP, recombinant expression of His-tagged wild-type and mutant enzymes in different Streptococcus pneumoniae strains. WIld-type PhpP expression in the Sp140 strain leads to increases in cell length when ZnSO4 is added to the medium, while cell length remains unchanged in strains Sp292 expressing enzyme mutant PhpP-D192A and Sp293 expressing enzyme mutant PhpP-D231A, phosphorylation pattern after induction of expression of GFP-tagged PhpP-wild-type (Sp140), GFP-tagged PhpP-D192A (Sp292) and GFP-tagged PhpP-D231A (Sp293) in DELTAphpP genetic background Streptococcus pneumoniae serotype 4

Protein Variants

Protein Variants Comment Organism
D192A site-directed mutagenesis, the mutant has abolished PhpP activity in vitro Streptococcus pneumoniae serotype 4
D231A site-directed mutagenesis, the mutant has abolished PhpP activity in vitro Streptococcus pneumoniae serotype 4
additional information generation of diverse mutant forms of the wild-type strain ATCC BAA-334 / TIGR4 or Rx1, introduction of specific mutations in the phpP and jag genes, construction of a phpP knockout strain and a strain overexpressing phpP, phenotypes, overview. The unencapsulated Rx1 phpP knockout strain is viable. The morphology of both, the unencapsulated phpP null mutant and the phpP overexpression strain, clearly demonstrated that PhpP participates in the regulation of cell division and has an opposite regulatory effect to that of Ser/Thr protein kinase, StkP. The phpP knockout strain is sensitive to elevated temperature and oxidative stress. Phenotype of a DELTAjag mutant, overview Streptococcus pneumoniae serotype 4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[a protein]-serine/threonine phosphate + H2O Streptococcus pneumoniae serotype 4
-
[a protein]-serine/threonine + phosphate
-
?
[a protein]-serine/threonine phosphate + H2O Streptococcus pneumoniae serotype 4 TIGR4
-
[a protein]-serine/threonine + phosphate
-
?
[a protein]-serine/threonine phosphate + H2O Streptococcus pneumoniae serotype 4 Rx1
-
[a protein]-serine/threonine + phosphate
-
?
[a protein]-serine/threonine phosphate + H2O Streptococcus pneumoniae serotype 4 ATCC BAA-334
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[a protein]-serine/threonine + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae serotype 4 Q8KY51
-
-
Streptococcus pneumoniae serotype 4 ATCC BAA-334 Q8KY51
-
-
Streptococcus pneumoniae serotype 4 Rx1 Q8KY51
-
-
Streptococcus pneumoniae serotype 4 TIGR4 Q8KY51
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant expression of His-tagged wild-type and mutant enzymes by nickel affinity chromatography Streptococcus pneumoniae serotype 4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[a protein]-serine/threonine phosphate + H2O
-
Streptococcus pneumoniae serotype 4 [a protein]-serine/threonine + phosphate
-
?
[a protein]-serine/threonine phosphate + H2O
-
Streptococcus pneumoniae serotype 4 TIGR4 [a protein]-serine/threonine + phosphate
-
?
[a protein]-serine/threonine phosphate + H2O
-
Streptococcus pneumoniae serotype 4 Rx1 [a protein]-serine/threonine + phosphate
-
?
[a protein]-serine/threonine phosphate + H2O
-
Streptococcus pneumoniae serotype 4 ATCC BAA-334 [a protein]-serine/threonine + phosphate
-
?
[protein DivIVA]-serine/threonine phosphate + H2O a protein substrate of Ser/Thr protein kinase (StkP), as recombinant FLAG-tagged protein Streptococcus pneumoniae serotype 4 [protein DivIVA]-serine/threonine + phosphate
-
?
[protein DivIVA]-serine/threonine phosphate + H2O a protein substrate of Ser/Thr protein kinase (StkP), as recombinant FLAG-tagged protein Streptococcus pneumoniae serotype 4 TIGR4 [protein DivIVA]-serine/threonine + phosphate
-
?
[protein DivIVA]-serine/threonine phosphate + H2O a protein substrate of Ser/Thr protein kinase (StkP), as recombinant FLAG-tagged protein Streptococcus pneumoniae serotype 4 Rx1 [protein DivIVA]-serine/threonine + phosphate
-
?
[protein DivIVA]-serine/threonine phosphate + H2O a protein substrate of Ser/Thr protein kinase (StkP), as recombinant FLAG-tagged protein Streptococcus pneumoniae serotype 4 ATCC BAA-334 [protein DivIVA]-serine/threonine + phosphate
-
?
[protein Jag]-serine/threonine phosphate + H2O i.e. EloR or Spr1851 protein, a cytoplasmic protein and putative RNA-binding protein Streptococcus pneumoniae serotype 4 [protein Jag]-serine/threonine + phosphate
-
?
[protein Jag]-serine/threonine phosphate + H2O i.e. EloR or Spr1851 protein, a cytoplasmic protein and putative RNA-binding protein Streptococcus pneumoniae serotype 4 TIGR4 [protein Jag]-serine/threonine + phosphate
-
?
[protein Jag]-serine/threonine phosphate + H2O i.e. EloR or Spr1851 protein, a cytoplasmic protein and putative RNA-binding protein Streptococcus pneumoniae serotype 4 Rx1 [protein Jag]-serine/threonine + phosphate
-
?
[protein Jag]-serine/threonine phosphate + H2O i.e. EloR or Spr1851 protein, a cytoplasmic protein and putative RNA-binding protein Streptococcus pneumoniae serotype 4 ATCC BAA-334 [protein Jag]-serine/threonine + phosphate
-
?
[protein LocZ]-serine/threonine phosphate + H2O a protein substrate of Ser/Thr protein kinase (StkP), as recombinant FLAG-tagged protein Streptococcus pneumoniae serotype 4 [protein locZ]-serine/threonine + phosphate
-
?
[protein LocZ]-serine/threonine phosphate + H2O a protein substrate of Ser/Thr protein kinase (StkP), as recombinant FLAG-tagged protein Streptococcus pneumoniae serotype 4 TIGR4 [protein locZ]-serine/threonine + phosphate
-
?
[protein LocZ]-serine/threonine phosphate + H2O a protein substrate of Ser/Thr protein kinase (StkP), as recombinant FLAG-tagged protein Streptococcus pneumoniae serotype 4 Rx1 [protein locZ]-serine/threonine + phosphate
-
?
[protein LocZ]-serine/threonine phosphate + H2O a protein substrate of Ser/Thr protein kinase (StkP), as recombinant FLAG-tagged protein Streptococcus pneumoniae serotype 4 ATCC BAA-334 [protein locZ]-serine/threonine + phosphate
-
?

Synonyms

Synonyms Comment Organism
PhpP
-
Streptococcus pneumoniae serotype 4
Ser/Thr protein phosphatase
-
Streptococcus pneumoniae serotype 4

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Streptococcus pneumoniae serotype 4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Streptococcus pneumoniae serotype 4

General Information

General Information Comment Organism
malfunction catalytic inactivation or absence of PhpP results in the hyperphosphorylation of Ser/Thr protein kinase (StkP) substrates and specific phenotypic changes, including sensitivity to environmental stresses and competence deficiency. The morphology of the DELTAphpP cells resembles the StkP overexpression phenotype and conversely, overexpression of PhpP results in cell elongation mimicking the stkP null phenotype. Proteomic analysis of the phpP knockout strain permits identification of a StkP/PhpP substrate, Spr1851, a putative RNA-binding protein homologous to Jag. Pneumococcal protein Jag is phosphorylated on Thr89. Inactivation of jag confers a phenotype similar to the phpP mutant strain. The unencapsulated Rx1 phpP knockout strain is viable. The morphology of both, the unencapsulated phpP null mutant and the phpP overexpression strain, clearly demonstrated that PhpP participates in the regulation of cell division and has an opposite regulatory effect to that of Ser/Thr protein kinase, StkP. The phpP knockout strain is sensitive to elevated temperature and oxidative stress Streptococcus pneumoniae serotype 4
additional information conserved residues D192 and D231 are essential for PhpP activity in vivo Streptococcus pneumoniae serotype 4
physiological function enzyme PhpP and StkP cooperatively regulate cell division of Streptococcus pneumoniae and phosphorylate putative RNA binding protein Jag Streptococcus pneumoniae serotype 4