Literature summary for 3.1.3.16 extracted from

Kennelly, P.J.; Oxenrider, K.A.; Leng, J.; Cantwell, J.S.; Zhao, N.
Identification of a serine/threonine-specific protein phosphatase from the archaebacterium Sulfolobus solfataricus (1993), J. Biol. Chem., 268, 6505-6510.

Search for more literature for 3.1.3.16

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	-	Saccharolobus solfataricus
diphosphate	10 mM, 93% inhibition	Saccharolobus solfataricus
EDTA	10 mM, 93% inhibition	Saccharolobus solfataricus
NaF	50 mM, 78% inhibition	Saccharolobus solfataricus
phosphate	10 mM, 76% inhibition	Saccharolobus solfataricus
ZnSO4	1 mM, 91% inhibition	Saccharolobus solfataricus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	stimulates	Saccharolobus solfataricus
Mg2+	stimulates	Saccharolobus solfataricus
Mn2+	stimulates	Saccharolobus solfataricus
additional information	Ca2+, Cu2+, or Zn2+ have little or no stimulatory effect	Saccharolobus solfataricus
Ni2+	stimulates	Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	gel filtration	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	typical specific activities observed in the presence of Mn2+ are on the order of 40-120 pmol of phosphate released from casein per min per mg of soluble extract protein	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
a phosphoprotein + H2O	the enzyme is specific from serinel/threonine-phosphorylated proteins including casein, histone H2a, phosphorylase kinase, or glycogen phosphorylase. The enzyme does not display detectable protein phosphatase activity toward a number of tyrosine-phosphorylated substrates	Saccharolobus solfataricus	a protein + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
protein phosphatase	-	Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
80	-	the enzyme is stable to heating for several min at temperatures up to 80°C	Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	casein as substrate	Saccharolobus solfataricus

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.5	-	virtually no protein phosphatase activity can be detected at pH values below 4.5	Saccharolobus solfataricus

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Release 2023.1 (January 2023)