Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.16 extracted from

  • Xu, Y.; Chen, Y.; Zhang, P.; Jeffrey, P.D.; Shi, Y.
    Structure of a protein phosphatase 2A holoenzyme: insights into B55-mediated Tau dephosphorylation (2008), Mol. Cell, 31, 873-885.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in baculovirus-infected insect cells and in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, using 7-10% (w/v) PEG35000 and 0.1-0.15 M sodium citrate (pH 5.5) Homo sapiens

Protein Variants

Protein Variants Comment Organism
D197K the Balpha mutant shows impaired ability to facilitate the dephosphorylation of phosphorylated Tau Homo sapiens
E27R the Balpha mutant shows impaired ability to facilitate the dephosphorylation of phosphorylated Tau Homo sapiens
K345E the Balpha mutant exhibits similar activity as the wild type Balpha regulatory subunit of PP2A Homo sapiens
K345E the Balpha mutant shows impaired ability to facilitate the dephosphorylation of phosphorylated Tau Homo sapiens
K48E the Balpha mutant shows impaired ability to facilitate the dephosphorylation of phosphorylated Tau Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
1 * 65000 + 1 * 36000 + 1 * ?, the PP2A core enzyme comprises a 65000 Da scaffold subunit and a 36000 Da catalytic subunit, to gain full activity toward specific substrates the PP2A core enzyme interacts with a variable regulatory subunit to form a heterotrimeric holoenzyme Homo sapiens
65000
-
1 * 65000 + 1 * 36000 + 1 * ?, the PP2A core enzyme comprises a 65000 Da scaffold subunit and a 36000 Da catalytic subunit, to gain full activity toward specific substrates the PP2A core enzyme interacts with a variable regulatory subunit to form a heterotrimeric holoenzyme Homo sapiens
155000
-
PP2A holoenzyme, X-ray crystallography Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification methylation Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphorylated Tau + H2O the Balpha regulatory subunit of PP2A facilitates dephosphorylation of the phosphorylated Tau Homo sapiens Tau + phosphate
-
?

Subunits

Subunits Comment Organism
heterotrimer 1 * 65000 + 1 * 36000 + 1 * ?, the PP2A core enzyme comprises a 65000 Da scaffold subunit and a 36000 Da catalytic subunit, to gain full activity toward specific substrates the PP2A core enzyme interacts with a variable regulatory subunit to form a heterotrimeric holoenzyme Homo sapiens

Synonyms

Synonyms Comment Organism
Balpha
-
Homo sapiens
PP2A
-
Homo sapiens
protein phosphatase 2A
-
Homo sapiens