Cloned (Comment) | Organism |
---|---|
expressed in baculovirus-infected insect cells and in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, using 7-10% (w/v) PEG35000 and 0.1-0.15 M sodium citrate (pH 5.5) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D197K | the Balpha mutant shows impaired ability to facilitate the dephosphorylation of phosphorylated Tau | Homo sapiens |
E27R | the Balpha mutant shows impaired ability to facilitate the dephosphorylation of phosphorylated Tau | Homo sapiens |
K345E | the Balpha mutant exhibits similar activity as the wild type Balpha regulatory subunit of PP2A | Homo sapiens |
K345E | the Balpha mutant shows impaired ability to facilitate the dephosphorylation of phosphorylated Tau | Homo sapiens |
K48E | the Balpha mutant shows impaired ability to facilitate the dephosphorylation of phosphorylated Tau | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
1 * 65000 + 1 * 36000 + 1 * ?, the PP2A core enzyme comprises a 65000 Da scaffold subunit and a 36000 Da catalytic subunit, to gain full activity toward specific substrates the PP2A core enzyme interacts with a variable regulatory subunit to form a heterotrimeric holoenzyme | Homo sapiens |
65000 | - |
1 * 65000 + 1 * 36000 + 1 * ?, the PP2A core enzyme comprises a 65000 Da scaffold subunit and a 36000 Da catalytic subunit, to gain full activity toward specific substrates the PP2A core enzyme interacts with a variable regulatory subunit to form a heterotrimeric holoenzyme | Homo sapiens |
155000 | - |
PP2A holoenzyme, X-ray crystallography | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | methylation | Homo sapiens |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphorylated Tau + H2O | the Balpha regulatory subunit of PP2A facilitates dephosphorylation of the phosphorylated Tau | Homo sapiens | Tau + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | 1 * 65000 + 1 * 36000 + 1 * ?, the PP2A core enzyme comprises a 65000 Da scaffold subunit and a 36000 Da catalytic subunit, to gain full activity toward specific substrates the PP2A core enzyme interacts with a variable regulatory subunit to form a heterotrimeric holoenzyme | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Balpha | - |
Homo sapiens |
PP2A | - |
Homo sapiens |
protein phosphatase 2A | - |
Homo sapiens |