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Literature summary for 3.1.3.16 extracted from
- Importance of a surface hydrophobic pocket on protein phosphatase-1 catalytic subunit in recognizing cellular regulators (2005), J. Biol. Chem., 280, 15903-15911.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant PP1alpha subunits in Escherichia coli strain BL21 | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C291R | site-directed mutagenesis, mutation of a hydrophobic surface pocket residue of subunit PP1alpha, the mutant shows reduced activity and impaired binding to protein phosphatase inhibitor-1 and protein phosphatase inhibitor-2, as well as to the neuronal regulatory subunit neurabin-I, and GM, the skeletal muscle glycogen-targeting subunit | Homo sapiens |
| L289R | site-directed mutagenesis, mutation of a hydrophobic surface pocket residue of subunit PP1alpha, the mutant shows reduced activity and reduced sensitivity to protein phosphatase inhibitor-1 and protein phosphatase inhibitor-2, as well as to the neuronal regulatory subunit neurabin-I, and GM, the skeletal muscle glycogen-targeting subunit | Homo sapiens |
| M290K | site-directed mutagenesis, mutation of a hydrophobic surface pocket residue of subunit PP1alpha, the mutant shows reduced activity and reduced sensitivity to protein phosphatase inhibitor-1 and protein phosphatase inhibitor-2, as well as to the neuronal regulatory subunit neurabin-I, and GM, the skeletal muscle glycogen-targeting subunit | Homo sapiens |
| additional information | construction of a C-terminally truncated PP1alpha subunit and a chimeric PP1alpha subunit containing the C-terminus of PP2A | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | IC50 of inhibitors for mutant PP1alpha catalytic subunits, overview | Homo sapiens | |
| neurabin-I | IC50 for the wild-type catalytic subunit PP1alpha is 1.75 nM | Homo sapiens | |
| okadaic acid | IC50 for the wild-type catalytic subunit PP1alpha is 157 nM | Homo sapiens |  |
| Protein phosphatase inhibitor-1 | i.e. I-1, specific inhibition of PP1, contains a consensus RVxF motif sequence, IC50 for the wild-type catalytic subunit PP1alpha is 25 nM | Homo sapiens | |
| protein phosphatase inhibitor-2 | i.e. I-2, specific inhibition of PP1, contains a consensus RVxF motif sequence, IC50 for the wild-type catalytic subunit PP1alpha is 2 nM | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | PP1 contains a surface hydrophobic pocket on the catalytic subunit which is important in recognizing cellular regulators via their consensus RVxF motifs, regulation of the enzyme, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant PP1alpha subunits from Escherichia coli strain BL21 performing multiple chromatographic steps including affinity chromatography on MLCR- and heparin-resins | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| neuron | - |
Homo sapiens | - |
| skeletal muscle | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | PP1 contains a surface hydrophobic pocket on the catalytic subunit which is important in recognizing cellular regulators via their consensus RVxF motifs, regulation of the enzyme, overview | Homo sapiens | ? | - |
? | |
| phosphorylated myelin basic protein + H2O | - |
Homo sapiens | myelin basic protein + phosphate | - |
? | |
| phosphorylated phosphorylase a + H2O | - |
Homo sapiens | phosphorylase a + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | association of catalytic subunit PP1alpha with regulatory subunits, e.g. the neuronal regulatory subunit neurabin-I, or GM, the skeletal muscle glycogen-targeting subunit, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | EC 3.1.3.53, myosin-light-chain-phosphatase | Homo sapiens |
| PP1 | - |
Homo sapiens |
| PP2A | - |
Homo sapiens |
| protein phosphatase-1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00000175 | - |
IC50 for the wild-type catalytic subunit PP1alpha is 1.75 nM | Homo sapiens | neurabin-I | |
| 0.000002 | - |
i.e. I-2, specific inhibition of PP1, contains a consensus RVxF motif sequence, IC50 for the wild-type catalytic subunit PP1alpha is 2 nM | Homo sapiens | protein phosphatase inhibitor-2 | |
| 0.000025 | - |
i.e. I-1, specific inhibition of PP1, contains a consensus RVxF motif sequence, IC50 for the wild-type catalytic subunit PP1alpha is 25 nM | Homo sapiens | Protein phosphatase inhibitor-1 | |
| 0.000157 | - |
IC50 for the wild-type catalytic subunit PP1alpha is 157 nM | Homo sapiens | okadaic acid | |
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