Cloned (Comment) | Organism |
---|---|
the PP5 catalytic domain comprising residues 169-499 is expressed as His-tagged maltose-binding protein fusion protein containing a tobacco etch virus cleavage site in Escherichia coli strain BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged PP5 catalytic domain, hanging drop vapour diffusion method, 22°C, 20 mg/ml protein in 40 mM Tris, pH 7.5, is mixed with an equal volume of reservoir solution containing 45-60% 2-methyl-2,4-pentanediol, X-ray diffraction structure determination and analysis at 1.6 A resolution | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | trace amounts | Homo sapiens | |
Mn2+ | 1 ion per enzyme molecule, involved in metal-mediated substrate hydrolysis | Homo sapiens | |
Zn2+ | trace amounts | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | PP5 affects many signaling pathways regulating cell growth and cellular stress response | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P36873 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged PP5 catalytic domain maltose-binding protein fusion protein from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, after cleavage of the fusion protein the His-tagged enzyme is further purified by anion exchange chromatography | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate | structural basis for the catalytic activity, the catalytic motif Asp271-metal1:metal2-water1-His427-His304-Asp274 requires precise substrate positioning, catalytic mechanism, metal-mediated substrate hydrolysis | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PP5 affects many signaling pathways regulating cell growth and cellular stress response | Homo sapiens | ? | - |
? | |
additional information | the enzyme performs metal-mediated substrate hydrolysis | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PP5 | - |
Homo sapiens |
serine/threonine protein phosphatase-5 | - |
Homo sapiens |