Literature summary for 3.1.3.16 extracted from

Yang, J.; Roe, S.M.; Cliff, M.J.; Williams, M.A.; Ladbury, J.E.; Cohen, P.T.; Barford, D.
Molecular basis for TPR domain-mediated regulation of protein phosphatase 5 (2005), EMBO J., 24, 1-10.

Activating Compound

Activating Compound	Comment	Organism	Structure
arachidonic acid	activation mechanism by prompting an alternate conformation of the TPR domain, destabilising the TPR domain-enzyme interface	Homo sapiens
Hsp90	heat shock protein, activation mechanism via interruption of the TPR domain interactions	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression of Ppp5 in Escherichia coli strain B834	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, under-oil-batch method, 21 mg/ml protein in solution is mixed with an eqal volume of crystallization buffer containing 10% w/v PEG 6000, 4% 2-methyl-2,4-pentanediol, 0.1 M Na-HEPES, pH 8.5, 3 mM DTT, under silicon oil at 14°C, cryoprotection buffer contains 13% w/v PEG 6000, 18% 2-methyl-2,4-pentanediol, 0.075 M NaHEPES, pH 8.5, X-ray diffraction structure determination and analysis at 2.9 A resolution, molecular replacement, modeling	Homo sapiens

Crystallization (Comment)

Organism

purified recombinant enzyme, under-oil-batch method, 21 mg/ml protein in solution is mixed with an eqal volume of crystallization buffer containing 10% w/v PEG 6000, 4% 2-methyl-2,4-pentanediol, 0.1 M Na-HEPES, pH 8.5, 3 mM DTT, under silicon oil at 14°C, cryoprotection buffer contains 13% w/v PEG 6000, 18% 2-methyl-2,4-pentanediol, 0.075 M NaHEPES, pH 8.5, X-ray diffraction structure determination and analysis at 2.9 A resolution, molecular replacement, modeling

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	Ppp5 is inhibited by an autoinhitory conformation formed by the N-terminal TPR domain and a C-terminal subdomain, structure and mechanism overview	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	-	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	Ppp5 is involved in signalling pathways that control cellular responses to stress, glucocorticoids, and DNA damage, molecular basis for N-terminal tetratricopeptide repeat domain TPR-mediated regulation of Ppp5	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant Ppp5 from Escherichia coli strain B834 by cation exchange, hydrophobic interaction, and nickel affinity chromatography and gel filtration	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate	Glu76 is involved in catalysis, active site structure	Homo sapiens	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	Ppp5 is involved in signalling pathways that control cellular responses to stress, glucocorticoids, and DNA damage, molecular basis for N-terminal tetratricopeptide repeat domain TPR-mediated regulation of Ppp5	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
More	molecular basis for N-terminal TPR domain-mediated regulation of Ppp5, overview	Homo sapiens

Synonyms

Synonyms	Comment	Organism
Ppp5	-	Homo sapiens
protein phosphatase 5	-	Homo sapiens
serine/threonine phosphatase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Homo sapiens