Literature summary for 3.1.3.16 extracted from

Nakai, C.; Thomas, J.A.
Properties of a phosphoprotein phosphatase from bovine heart with activity on glycogen synthase, phosphorylase, and histone (1974), J. Biol. Chem., 249, 6459-6467.

Activating Compound

Activating Compound	Comment	Organism
ADP	1.4 mM, 30°C, pH 7.5, 104% relative activity with histone as substrate	Bos taurus
AMP	1.4 mM, 30°C, pH 7.5, 102% relative activity with histone as substrate	Bos taurus
ATP	1.4 mM, 30°C, pH 7.5, 107% relative activity with histone as substrate	Bos taurus
bovine serum albumin	10 mg/ml, 30°C, pH 7.5, 112% relative activity with histone as substrate	Bos taurus
CaCl2	14 mM, 30°C, pH 7.5, relative activity 126% with glycogen synthase D as substrate and 232% with histone as substrate compared to no addition	Bos taurus
cAMP	1 mM, 30°C, pH 7.5, 115% relative activity with glycogen synthase D as substrate	Bos taurus
D-glucosamine 6-phosphate	1.4 mM, 30°C, pH7.5, relative activity 109% with glycogen synthase D as substrate, 117% with histone as substrate, and 102% with phosphorylase a as substrate compared to no addition	Bos taurus
D-glucose	1.4 mM, 30°C, pH7.5, relative activity 109% with glycogen synthase D as substrate, 101% with histone as substrate, and 106% with phosphorylase a as substrate compared to no addition	Bos taurus
D-glucose 6-phosphate	1.4 mM, 30°C, pH 7.5, 109% relative activity with phosphorylase a as substrate	Bos taurus
EDTA	14 mM, 30°C, pH 7.5, 175% relative activity with histone as substrate	Bos taurus
EGTA	14 mM, 30°C, pH 7.5, 127% relative activity with histone as substrate	Bos taurus
gelatin	1 mg/ml, 30°C, pH 7.5, 128% relative activity with glycogen synthase D as substrate, with 10 mg/ml 110% relative activity	Bos taurus
MgCl2	14 mM, 30°C, pH 7.5, relative activity 256% with glycogen synthase D as substrate and 238% with histone as substrate compared to no addition	Bos taurus
MnCl2	14 mM, 30°C, pH 7.5, relative activity 361% with glycogen synthase D as substrate and 280% with histone as substrate compared to no addition	Bos taurus
additional information	study of effects of D-glucose 6-phosphate on activating compounds	Bos taurus
additional information	study of effects of MgCl2 in presence of AMP, ADP, ATP, UMP, UDP, UTP, or citrate	Bos taurus
Na2SO4	14 mM, 30°C, pH 7.5, relative activity 152% with histone as substrate compared to no addition	Bos taurus
NaCl	14 mM, 30°C, pH 7.5, relative activity 102% with glycogen synthase D as substrate and 108% with histone as substrate compared to no addition	Bos taurus
rabbit liver glycogen	1 mg/ml, 30°C, pH 7.5, 119% relative activity with histone as substrate	Bos taurus
UMP	1.4 mM, 30°C, pH 7.5, 102% relative activity with histone as substrate	Bos taurus
UTP	1.4 mM, 30°C, pH 7.5, 108% relative activity with histone as substrate	Bos taurus

Inhibitors

Inhibitors	Comment	Organism
ADP	1.4 mM, 30°C, pH 7.5, 55% remaining activity with glycogen synthase D as substrate and 12% with phosphorylase a	Bos taurus
AMP	1.4 mM, 30°C, pH 7.5, 55% remaining activity with glycogen synthase D as substrate and 10% with phosphorylase a	Bos taurus
ATP	1.4 mM, 30°C, pH 7.5, 47% remaining activity with glycogen synthase D as substrate and 15% with phosphorylase a	Bos taurus
bovine serum albumin	1 mg/ml 30°C, pH 7.5, 95% remaining activity with glycogen synthase D and 92% with phosphorylase, in presence of 10 mg/ml 79% remaining activity with phosphorylase a as substrate	Bos taurus
CaCl2	14 mM, 30°C, pH 7.5, with phosphorylase a as substrate, 50% remaining activity	Bos taurus
cAMP	1 mM, 30°C, pH 7.5, 83% remaining activity with histone as substrate and 83% with phosphorylase a	Bos taurus
citrate	1.4 mM, 30°C, pH 7.5, 68% remaining activity with glycogen synthase D as substrate	Bos taurus
EDTA	14 mM, 30°C, pH 7.5, 23% remaining activity with glycogen synthase D as substrate and 35% with phosphorylase a	Bos taurus
EGTA	14 mM, 30°C, pH 7.5, 55% remaining activity with glycogen synthase D as substrate and 83% with phosphorylase a	Bos taurus
gelatin	1 mg/ml, 30°C, pH 7.5, 92% remaining activity with histone as substrate, and 31% withe phosphorylase a, in presence of 10 mg/ml 10% remaining activity with phohsphorylase a	Bos taurus
histone	competitive when glycogen synthase D is the substrate, increases the Km-value for glycogen synthase D 4fold, but shows mixed type inhibition when phosphorylase a acts as substrate with an 1.4fold increase of Km	Bos taurus
MgCl2	14 mM, 30°C, pH 7.5, with phosphorylase a as substrate, 60% remaining activity	Bos taurus
MnCl2	14 mM, 30°C, pH 7.5, with phosphorylase a as substrate, 51% remaining activity	Bos taurus
additional information	study of effects of D-glucose 6-phosphate on inhibitory compounds; study of effects of MgCl2 in presence of AMP, ADP, ATP, UMP, UDP, UTP, or citrate	Bos taurus
Na2HPO4	14 mM, 30°C, pH 7.5, no activity with glycogen synthase D as substrate, remaining activity 79% with histone as substrate, and 64% with phosphorylase a as substrate	Bos taurus
Na2P2O7	14 mM, 30°C, pH 7.5, remaining activity 6% with glycogen synthase D as substrate, 15% with histone as substrate, and 8% with phosphorylase a as substrate	Bos taurus
Na2SO4	14 mM, 30°C, pH 7.5, remaining activity 53% with glycogen synthase D as substrate and 32% with phosphorylase a as substrate	Bos taurus
NaCl	14 mM, 30°C, pH 7.5, with phosphorylase a as substrate, 83% remaining activity	Bos taurus
NaF	14 mM, 30°C, pH 7.5, remaining activity 31% with glycogen synthase D as substrate, 67% with histone as substrate, and 37% with phosphorylase a as substrate	Bos taurus
NaHCO3	14 mM, 30°C, pH 7.5, remaining activity 49% with glycogen synthase D as substrate, 79% with histone as substrate, and 64% with phosphorylase a as substrate	Bos taurus
NaNO3	14 mM, 30°C, pH 7.5, remaining activity 70% with glycogen synthase D as substrate, 83% with histone as substrate, and 78% with phosphorylase a as substrate	Bos taurus
phosphorylase a	mixed type inhibitor with glycogen synthase d as substrate, increases Km 2fold with only small decearse in vmax	Bos taurus
rabbit liver glycogen	1 mg/ml 30°C, pH 7.5, 88% remaining activity with glycogen synthase D and 37% remaining activity with 10 mg/ml inhibitor	Bos taurus
UDP	1.4 mM, 30°C, pH 7.5, 52% remaining activity with glycogen synthase D as substrate and 20% with phosphorylase a	Bos taurus
UMP	1.4 mM, 30°C, pH 7.5, 58% remaining activity with glycogen synthase D as substrate and 16% with phosphorylase a	Bos taurus
UTP	1.4 mM, 30°C, pH 7.5, 44% remaining activity with glycogen synthase D as substrate and 21% with phosphorylase a	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics with glycogen synthase D, phosphorylase a, and histone in presence of no, 10, 35, and 100 mM MgCl2, expressed as concentrations of [32P] phosphate	Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
phosphoprotein + H2O	Bos taurus	-	protein + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
150fold purified, ratio of activities with glycogen synthase D, phosphorylase a, and histone as substrates are unchanged during purification, final enzyme preparation is also active on phosphorylase kinase and casein, no activity on p-nitrophenyl phosphate, when assayed at pH 7.5 or at pH 10.5	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
heart	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
glycogen synthase D + H2O	-	Bos taurus	glycogen synthase I + phosphate	-	r
additional information	no activity on p-nitrophenyl phosphate, when assayed at pH 7.5 or at pH 10.5	Bos taurus	?	-	?
phosphocasein + H2O	-	Bos taurus	casein + phosphate	-	?
phosphoprotein + H2O	-	Bos taurus	protein + phosphate	-	?
phosphorylase a + H2O	-	Bos taurus	phosphorylase b + phosphate	-	r
phosphorylated histone + H2O	-	Bos taurus	histone + phosphate	-	?
phosphorylated phosphorylase kinase + H2O	-	Bos taurus	phosphorylase kinase + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
general phosphoprotein phosphatase	-	Bos taurus