Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | 1.4 mM, 30°C, pH 7.5, 104% relative activity with histone as substrate | Bos taurus | |
AMP | 1.4 mM, 30°C, pH 7.5, 102% relative activity with histone as substrate | Bos taurus | |
ATP | 1.4 mM, 30°C, pH 7.5, 107% relative activity with histone as substrate | Bos taurus | |
bovine serum albumin | 10 mg/ml, 30°C, pH 7.5, 112% relative activity with histone as substrate | Bos taurus | |
CaCl2 | 14 mM, 30°C, pH 7.5, relative activity 126% with glycogen synthase D as substrate and 232% with histone as substrate compared to no addition | Bos taurus | |
cAMP | 1 mM, 30°C, pH 7.5, 115% relative activity with glycogen synthase D as substrate | Bos taurus | |
D-glucosamine 6-phosphate | 1.4 mM, 30°C, pH7.5, relative activity 109% with glycogen synthase D as substrate, 117% with histone as substrate, and 102% with phosphorylase a as substrate compared to no addition | Bos taurus | |
D-glucose | 1.4 mM, 30°C, pH7.5, relative activity 109% with glycogen synthase D as substrate, 101% with histone as substrate, and 106% with phosphorylase a as substrate compared to no addition | Bos taurus | |
D-glucose 6-phosphate | 1.4 mM, 30°C, pH 7.5, 109% relative activity with phosphorylase a as substrate | Bos taurus | |
EDTA | 14 mM, 30°C, pH 7.5, 175% relative activity with histone as substrate | Bos taurus | |
EGTA | 14 mM, 30°C, pH 7.5, 127% relative activity with histone as substrate | Bos taurus | |
gelatin | 1 mg/ml, 30°C, pH 7.5, 128% relative activity with glycogen synthase D as substrate, with 10 mg/ml 110% relative activity | Bos taurus | |
MgCl2 | 14 mM, 30°C, pH 7.5, relative activity 256% with glycogen synthase D as substrate and 238% with histone as substrate compared to no addition | Bos taurus | |
MnCl2 | 14 mM, 30°C, pH 7.5, relative activity 361% with glycogen synthase D as substrate and 280% with histone as substrate compared to no addition | Bos taurus | |
additional information | study of effects of D-glucose 6-phosphate on activating compounds | Bos taurus | |
additional information | study of effects of MgCl2 in presence of AMP, ADP, ATP, UMP, UDP, UTP, or citrate | Bos taurus | |
Na2SO4 | 14 mM, 30°C, pH 7.5, relative activity 152% with histone as substrate compared to no addition | Bos taurus | |
NaCl | 14 mM, 30°C, pH 7.5, relative activity 102% with glycogen synthase D as substrate and 108% with histone as substrate compared to no addition | Bos taurus | |
rabbit liver glycogen | 1 mg/ml, 30°C, pH 7.5, 119% relative activity with histone as substrate | Bos taurus | |
UMP | 1.4 mM, 30°C, pH 7.5, 102% relative activity with histone as substrate | Bos taurus | |
UTP | 1.4 mM, 30°C, pH 7.5, 108% relative activity with histone as substrate | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 1.4 mM, 30°C, pH 7.5, 55% remaining activity with glycogen synthase D as substrate and 12% with phosphorylase a | Bos taurus | |
AMP | 1.4 mM, 30°C, pH 7.5, 55% remaining activity with glycogen synthase D as substrate and 10% with phosphorylase a | Bos taurus | |
ATP | 1.4 mM, 30°C, pH 7.5, 47% remaining activity with glycogen synthase D as substrate and 15% with phosphorylase a | Bos taurus | |
bovine serum albumin | 1 mg/ml 30°C, pH 7.5, 95% remaining activity with glycogen synthase D and 92% with phosphorylase, in presence of 10 mg/ml 79% remaining activity with phosphorylase a as substrate | Bos taurus | |
CaCl2 | 14 mM, 30°C, pH 7.5, with phosphorylase a as substrate, 50% remaining activity | Bos taurus | |
cAMP | 1 mM, 30°C, pH 7.5, 83% remaining activity with histone as substrate and 83% with phosphorylase a | Bos taurus | |
citrate | 1.4 mM, 30°C, pH 7.5, 68% remaining activity with glycogen synthase D as substrate | Bos taurus | |
EDTA | 14 mM, 30°C, pH 7.5, 23% remaining activity with glycogen synthase D as substrate and 35% with phosphorylase a | Bos taurus | |
EGTA | 14 mM, 30°C, pH 7.5, 55% remaining activity with glycogen synthase D as substrate and 83% with phosphorylase a | Bos taurus | |
gelatin | 1 mg/ml, 30°C, pH 7.5, 92% remaining activity with histone as substrate, and 31% withe phosphorylase a, in presence of 10 mg/ml 10% remaining activity with phohsphorylase a | Bos taurus | |
histone | competitive when glycogen synthase D is the substrate, increases the Km-value for glycogen synthase D 4fold, but shows mixed type inhibition when phosphorylase a acts as substrate with an 1.4fold increase of Km | Bos taurus | |
MgCl2 | 14 mM, 30°C, pH 7.5, with phosphorylase a as substrate, 60% remaining activity | Bos taurus | |
MnCl2 | 14 mM, 30°C, pH 7.5, with phosphorylase a as substrate, 51% remaining activity | Bos taurus | |
additional information | study of effects of D-glucose 6-phosphate on inhibitory compounds; study of effects of MgCl2 in presence of AMP, ADP, ATP, UMP, UDP, UTP, or citrate | Bos taurus | |
Na2HPO4 | 14 mM, 30°C, pH 7.5, no activity with glycogen synthase D as substrate, remaining activity 79% with histone as substrate, and 64% with phosphorylase a as substrate | Bos taurus | |
Na2P2O7 | 14 mM, 30°C, pH 7.5, remaining activity 6% with glycogen synthase D as substrate, 15% with histone as substrate, and 8% with phosphorylase a as substrate | Bos taurus | |
Na2SO4 | 14 mM, 30°C, pH 7.5, remaining activity 53% with glycogen synthase D as substrate and 32% with phosphorylase a as substrate | Bos taurus | |
NaCl | 14 mM, 30°C, pH 7.5, with phosphorylase a as substrate, 83% remaining activity | Bos taurus | |
NaF | 14 mM, 30°C, pH 7.5, remaining activity 31% with glycogen synthase D as substrate, 67% with histone as substrate, and 37% with phosphorylase a as substrate | Bos taurus | |
NaHCO3 | 14 mM, 30°C, pH 7.5, remaining activity 49% with glycogen synthase D as substrate, 79% with histone as substrate, and 64% with phosphorylase a as substrate | Bos taurus | |
NaNO3 | 14 mM, 30°C, pH 7.5, remaining activity 70% with glycogen synthase D as substrate, 83% with histone as substrate, and 78% with phosphorylase a as substrate | Bos taurus | |
phosphorylase a | mixed type inhibitor with glycogen synthase d as substrate, increases Km 2fold with only small decearse in vmax | Bos taurus | |
rabbit liver glycogen | 1 mg/ml 30°C, pH 7.5, 88% remaining activity with glycogen synthase D and 37% remaining activity with 10 mg/ml inhibitor | Bos taurus | |
UDP | 1.4 mM, 30°C, pH 7.5, 52% remaining activity with glycogen synthase D as substrate and 20% with phosphorylase a | Bos taurus | |
UMP | 1.4 mM, 30°C, pH 7.5, 58% remaining activity with glycogen synthase D as substrate and 16% with phosphorylase a | Bos taurus | |
UTP | 1.4 mM, 30°C, pH 7.5, 44% remaining activity with glycogen synthase D as substrate and 21% with phosphorylase a | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics with glycogen synthase D, phosphorylase a, and histone in presence of no, 10, 35, and 100 mM MgCl2, expressed as concentrations of [32P] phosphate | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoprotein + H2O | Bos taurus | - |
protein + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
150fold purified, ratio of activities with glycogen synthase D, phosphorylase a, and histone as substrates are unchanged during purification, final enzyme preparation is also active on phosphorylase kinase and casein, no activity on p-nitrophenyl phosphate, when assayed at pH 7.5 or at pH 10.5 | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycogen synthase D + H2O | - |
Bos taurus | glycogen synthase I + phosphate | - |
r | |
additional information | no activity on p-nitrophenyl phosphate, when assayed at pH 7.5 or at pH 10.5 | Bos taurus | ? | - |
? | |
phosphocasein + H2O | - |
Bos taurus | casein + phosphate | - |
? | |
phosphoprotein + H2O | - |
Bos taurus | protein + phosphate | - |
? | |
phosphorylase a + H2O | - |
Bos taurus | phosphorylase b + phosphate | - |
r | |
phosphorylated histone + H2O | - |
Bos taurus | histone + phosphate | - |
? | |
phosphorylated phosphorylase kinase + H2O | - |
Bos taurus | phosphorylase kinase + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
general phosphoprotein phosphatase | - |
Bos taurus |