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Literature summary for 3.1.3.16 extracted from
- Purification and characterization of a divalent cation-independent, spermine-stimulated protein phosphatase from bovine kidney mitochondria (1987), J. Biol. Chem., 262, 5133-5138.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Bos taurus |  |
| ATP | - |
Bos taurus | |
| dephospho-CoA | - |
Bos taurus | |
| diphosphate | - |
Bos taurus | |
| F- | - |
Bos taurus | |
| guanosine 5'-(beta,gamma-imido)triphosphate | - |
Bos taurus | |
| isobutyryl-CoA | - |
Bos taurus | |
| isovaleryl-CoA | - |
Bos taurus | |
| Nucleoside mono-, di-, and triphosphates | e.g. GTP, GDP, ATP, ADP | Bos taurus | |
| phosphate | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Bos taurus | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50 mM imidazole chloride buffer, pH 7.3, 1 mM EDTA, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM dithiothreitol, 1.4 M NaCl, 10% glycerol, 8 months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphocasein + H2O | - |
Bos taurus | casein + phosphate | - |
? | |
| phosphoproteins + H2O | - |
Bos taurus | proteins + phosphate | - |
? | |
| phosphorylase a + H2O | - |
Bos taurus | phosphorylase b + phosphate | - |
? | |
| phosphorylated BCKDH complex + H2O | i.e. branched-chain alpha-keto acid dehydrogenase, phosphorylated | Bos taurus | BCKDH complex + phosphate | - |
? | |
| phosphorylated pyruvate dehydrogenase + H2O | - |
Bos taurus | pyruvate dehydrogenase + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | phosphatase SP | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | 7.1 | - |
Bos taurus |
| 7.6 | 8 | presence of spermine | Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | 7.6 | inactive above in absence of spermine | Bos taurus |
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