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Literature summary for 3.1.3.15 extracted from

  • Kulis-Horn, R.; Rueckert, C.; Kalinowski, J.; Persicke, M.
    Sequence-based identification of inositol monophosphatase-like histidinol-phosphate phosphatases (HisN) in Corynebacterium glutamicum, Actinobacteria, and beyond (2017), BMC Microbiol., 17, 161 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.15

Activating Compound

Activating Compound Comment Organism Structure
L-histidinol about 5% activation at 10 mM and above. Cg0911 is positively feedback regulated by L-histidinol Corynebacterium glutamicum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Corynebacterium glutamicum
gene cg0911 or cgl0800, sequence comparisons Corynebacterium glutamicum
gene hisN or cg0910, sequence comparisons Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
additional information generation of a DELTAhisN single mutant, a DELTAhisN DELTAcg0911 double mutant, and a quintuple mutant, lacking hisN and all its paralogues, phenotypes, overview. No further reduction of growth of the DELTAhisN DELTAcg0911 double or the DELTAhisN DELTAcg0911 DELTAimpA DELTAsuhB DELTAcysQ quintuple mutant as compared to the DELTAhisN single mutant. Supplementation with L-histidine results in the same growth of all mutants. Expression of impA, suhB or cysQ does not improve the growth of the DELTAhisN strain on minimal medium. Beside the complementation by hisN itself, a complementation of the DELTAhisN growth defect is only observed with cg0911. Although the complementation assay clearly demonstrates HolPase activity of the cg0911 gene product in vivo if expressed on a multiple copy plasmid, this activity does not account for L-histidine biosynthesis in a measurable degree if present in single copy under control of the native promotor Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
EDTA 10 mM, complete loss of activity; 10 mM, complete loss of activity Corynebacterium glutamicum
additional information no inhibitory effect is observed by L-histidine at up to 60 mM L-histidine, by phosphate at up to 0.25 mM, or by L-histidinol at 20 mM; no inhibitory effect of L-histidine is observed with concentrations up to 60 mM L-histidine Corynebacterium glutamicum
phosphate 40% inhibition at 0.375 mM Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0236
-
 L-histidinol phosphate pH 7.4, 30°C, Hill coefficient 1.47 Corynebacterium glutamicum
0.638
-
 L-histidinol phosphate pH 7.4, 30°C, Hill coefficient 1.47 Corynebacterium glutamicum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 20% of the activity with Mg2+ Corynebacterium glutamicum
Co2+ 20% activation compared to Mg2+ at 5 mM Corynebacterium glutamicum
Co2+ 47% of the activity with Mg2+ Corynebacterium glutamicum
Co2+ 50% activation compared to Mg2+ at 5 mM Corynebacterium glutamicum
Mg2+ activates, best divalent cation Corynebacterium glutamicum
Mg2+ assay in presence of 5 mM, Km value 0.65 mM, Hill coefficient 2.44 Corynebacterium glutamicum
Mn2+ 11% of the activity with Mg2+ Corynebacterium glutamicum
Mn2+ 78% of the activity with Mg2+ Corynebacterium glutamicum
Mn2+ 10% activation compared to Mg2+ at 5 mM Corynebacterium glutamicum
Mn2+ 75-80% activation compared to Mg2+ at 5 mM Corynebacterium glutamicum
additional information divalent cation is required Corynebacterium glutamicum
additional information no activating effect by Zn2+, Cu2+, Ca2+, Mi2+, and Fe2+ at 5 mM Corynebacterium glutamicum
additional information no activating effect on activity by Zn2+, Cu2+, Ca2+, Mi2+, and Fe2+ at 5 mM Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-histidinol phosphate + H2O Corynebacterium glutamicum
-
 L-histidinol + phosphate
-
 ?
L-histidinol phosphate + H2O Corynebacterium glutamicum LMG 3730
-
 L-histidinol + phosphate
-
 ?
L-histidinol phosphate + H2O Corynebacterium glutamicum ATCC 13032
-
 L-histidinol + phosphate
-
 ?
L-histidinol phosphate + H2O Corynebacterium glutamicum JCM 1318
-
 L-histidinol + phosphate
-
 ?
L-histidinol phosphate + H2O Corynebacterium glutamicum NCIMB 10025
-
 L-histidinol + phosphate
-
 ?
L-histidinol phosphate + H2O Corynebacterium glutamicum DSM 20300
-
 L-histidinol + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-
Corynebacterium glutamicum Q8NS79
-
-
Corynebacterium glutamicum Q8NS80
-
-
Corynebacterium glutamicum ATCC 13032 Q8NS79
-
-
Corynebacterium glutamicum ATCC 13032 Q8NS80
-
-
Corynebacterium glutamicum DSM 20300
-
-
-
Corynebacterium glutamicum DSM 20300 Q8NS79
-
-
Corynebacterium glutamicum DSM 20300 Q8NS80
-
-
Corynebacterium glutamicum JCM 1318 Q8NS79
-
-
Corynebacterium glutamicum JCM 1318 Q8NS80
-
-
Corynebacterium glutamicum LMG 3730 Q8NS79
-
-
Corynebacterium glutamicum LMG 3730 Q8NS80
-
-
Corynebacterium glutamicum NCIMB 10025 Q8NS79
-
-
Corynebacterium glutamicum NCIMB 10025 Q8NS80
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-histidinol phosphate + H2O
-
 Corynebacterium glutamicum L-histidinol + phosphate
-
 ?
L-histidinol phosphate + H2O low activity Corynebacterium glutamicum L-histidinol + phosphate
-
 ?
L-histidinol phosphate + H2O
-
 Corynebacterium glutamicum LMG 3730 L-histidinol + phosphate
-
 ?
L-histidinol phosphate + H2O low activity Corynebacterium glutamicum LMG 3730 L-histidinol + phosphate
-
 ?
L-histidinol phosphate + H2O
-
 Corynebacterium glutamicum ATCC 13032 L-histidinol + phosphate
-
 ?
L-histidinol phosphate + H2O low activity Corynebacterium glutamicum ATCC 13032 L-histidinol + phosphate
-
 ?
L-histidinol phosphate + H2O
-
 Corynebacterium glutamicum JCM 1318 L-histidinol + phosphate
-
 ?
L-histidinol phosphate + H2O low activity Corynebacterium glutamicum JCM 1318 L-histidinol + phosphate
-
 ?
L-histidinol phosphate + H2O
-
 Corynebacterium glutamicum NCIMB 10025 L-histidinol + phosphate
-
 ?
L-histidinol phosphate + H2O low activity Corynebacterium glutamicum NCIMB 10025 L-histidinol + phosphate
-
 ?
L-histidinol phosphate + H2O
-
 Corynebacterium glutamicum DSM 20300 L-histidinol + phosphate
-
 ?
L-histidinol phosphate + H2O low activity Corynebacterium glutamicum DSM 20300 L-histidinol + phosphate
-
 ?
additional information no substrate: 4-nitrophenyl phosphate Corynebacterium glutamicum ?
-
 ?
additional information no substrate: 4-nitrophenyl phosphate Corynebacterium glutamicum DSM 20300 ?
-
 ?

Synonyms

Synonyms Comment Organism
cg0910
-
 Corynebacterium glutamicum
Cg0911
-
 Corynebacterium glutamicum
Cgl0800
-
 Corynebacterium glutamicum
hisN
-
 Corynebacterium glutamicum
HolPase
-
 Corynebacterium glutamicum
IMPase-like HolPase
-
 Corynebacterium glutamicum
inositol monophosphatase-like HolPase
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Corynebacterium glutamicum
30
-
 recombinant enzyme Corynebacterium glutamicum
35 40
-
 Corynebacterium glutamicum
35 40 recombinant enzyme HisN Corynebacterium glutamicum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 maximal Cg0911 activity at 30°C and less than 5% of this activity remains at 40°C, profile overview Corynebacterium glutamicum
20 50 maximal HisN activity at 35-40°C, no activity at 50°C, profile overview Corynebacterium glutamicum
40
-
 less than 5% residual activity Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
 L-histidinol phosphate pH 7.4, 30°C Corynebacterium glutamicum
1.04
-
 L-histidinol phosphate pH 7.4, 30°C Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3 7.4 recombinant enzyme Corynebacterium glutamicum
7.4
-
-
 Corynebacterium glutamicum
8
-
-
 Corynebacterium glutamicum
8
-
 recombinant enzyme Corynebacterium glutamicum

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 recombinant enzyme, activity range, profile overview Corynebacterium glutamicum
7 10 recombinant enzyme, maximal Cg0911 activity is observed at around pH 8 and is only little reduced at pH 7.35, followed by a sharp loss in activity towards more acidic conditions. The drop in activity towards more alkaline conditions is less pronounced, the enzyme exhibits still 30% of its activity at around pH 10, profile overview Corynebacterium glutamicum

General Information

General Information Comment Organism
evolution distribution of Cg0911 orthologues within bacteria, sequences and structure motifs comparisons, phylogenetic analysis, overview Corynebacterium glutamicum
evolution distribution of HisN orthologues within bacteria, sequences and structure motifs comparisons, phylogenetic analysis, overview Corynebacterium glutamicum
malfunction deletion of hisN in Corynebacterium glutamicum results in pronounced L-histidine bradytrophy instead of complete auxotrophy. Growth of the DELTAhisN mutant is visible after several days of incubation on minimal medium plates without L-histidine. Addition of L-histidine abolishes the observed growth defect completely. Complementation of the DELTAhisN growth defect is observed with gene cg0911 Corynebacterium glutamicum
additional information importance of a highly conserved aspartate residue accompanied by several aromatic amino acid residues present in motif 5 for HolPase activity Corynebacterium glutamicum
physiological function gene cg0911 encodes an enzyme with HolPase activity, the hisN paralogue gene cg0911 can complement the growth defect of mutant DELTAhisN. Enzyme Cg0911 is positively feedback regulated by L-histidinol Corynebacterium glutamicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.002
-
 L-histidinol phosphate pH 7.4, 30°C, Hill coefficient 1.47 Corynebacterium glutamicum
44.1
-
 L-histidinol phosphate pH 7.4, 30°C, Hill coefficient 1.47 Corynebacterium glutamicum