Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Lactococcus lactis subsp. lactis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 25% (w/v) PEG 4000, 0.1 M sodium acetate (pH 4.6), and 0.2 M ammonium sulfate | Lactococcus lactis subsp. lactis |
Protein Variants | Comment | Organism |
---|---|---|
D17N | inactive | Lactococcus lactis subsp. lactis |
D228N | the mutant shows reduced catalytic efficiency and kcat is reduced by approximately 6000fold compared to the wild type enzyme | Lactococcus lactis subsp. lactis |
E115Q | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Lactococcus lactis subsp. lactis |
H230N | inactive | Lactococcus lactis subsp. lactis |
H42N | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Lactococcus lactis subsp. lactis |
R160A | inactive | Lactococcus lactis subsp. lactis |
R197M | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Lactococcus lactis subsp. lactis |
Y117A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Lactococcus lactis subsp. lactis |
Y117F | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Lactococcus lactis subsp. lactis |
Y157F | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Lactococcus lactis subsp. lactis |
Y161A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Lactococcus lactis subsp. lactis |
Y161F | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Lactococcus lactis subsp. lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
L-histidinol phosphate | mutant enzyme D228N, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
0.74 | - |
L-histidinol phosphate | mutant enzyme Y117F, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
1.3 | - |
L-histidinol phosphate | mutant enzyme H42N, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
1.5 | - |
L-histidinol phosphate | mutant enzyme R160M, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
1.7 | - |
L-histidinol phosphate | mutant enzyme E115Q, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
1.7 | - |
L-histidinol phosphate | mutant enzyme HY157F, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
1.9 | - |
L-histidinol phosphate | wild type enzyme, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
2.3 | - |
L-histidinol phosphate | mutant enzyme Y161F, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
2.5 | - |
L-histidinol phosphate | mutant enzyme Y161A, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
4.5 | - |
L-histidinol phosphate | mutant enzyme R197M, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
5.4 | - |
L-histidinol phosphate | mutant enzyme Y117A, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains iron in the active site | Lactococcus lactis subsp. lactis | |
additional information | the enzyme does not show any significant increases in catalytic activity when Zn2+, Mn2+, Co2+ or Ni2+ are added | Lactococcus lactis subsp. lactis | |
Zn2+ | contains Zn2+ in the active site | Lactococcus lactis subsp. lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-histidinol phosphate + H2O | Lactococcus lactis subsp. lactis | - |
L-histidinol + phosphate | - |
? | |
L-histidinol phosphate + H2O | Lactococcus lactis subsp. lactis IL1403 | - |
L-histidinol + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis subsp. lactis | Q02150 | - |
- |
Lactococcus lactis subsp. lactis IL1403 | Q02150 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity chromatography | Lactococcus lactis subsp. lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-histidinol phosphate + H2O | - |
Lactococcus lactis subsp. lactis | L-histidinol + phosphate | - |
? | |
L-histidinol phosphate + H2O | - |
Lactococcus lactis subsp. lactis IL1403 | L-histidinol + phosphate | - |
? | |
N-formyl-L-histidinol phosphate + H2O | the rate of hydrolysis of N-formyl-L-histidinol phosphate is less than 1% of the rate of hydrolysis of L-histidinol phosphate at pH 8.5 | Lactococcus lactis subsp. lactis | N-formyl-L-histidinol + phosphate | - |
? | |
N-formyl-L-histidinol phosphate + H2O | the rate of hydrolysis of N-formyl-L-histidinol phosphate is less than 1% of the rate of hydrolysis of L-histidinol phosphate at pH 8.5 | Lactococcus lactis subsp. lactis IL1403 | N-formyl-L-histidinol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HPP | - |
Lactococcus lactis subsp. lactis |
L-histidinol phosphate phosphatase | - |
Lactococcus lactis subsp. lactis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
L-histidinol phosphate | mutant enzyme D228N, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
0.62 | - |
L-histidinol phosphate | mutant enzyme H42N, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
0.74 | - |
L-histidinol phosphate | mutant enzyme R160M, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
31 | - |
L-histidinol phosphate | mutant enzyme E115Q, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
38 | - |
L-histidinol phosphate | mutant enzyme R197M, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
48 | - |
L-histidinol phosphate | mutant enzyme Y161A, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
53 | - |
L-histidinol phosphate | mutant enzyme Y117A, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
61 | - |
L-histidinol phosphate | mutant enzyme HY157F, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
110 | - |
L-histidinol phosphate | mutant enzyme Y117F, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
174 | - |
L-histidinol phosphate | wild type enzyme, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
180 | - |
L-histidinol phosphate | mutant enzyme Y161F, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
L-histidinol phosphate | mutant enzyme D228N, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
0.48 | - |
L-histidinol phosphate | mutant enzyme H42N, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
0.49 | - |
L-histidinol phosphate | mutant enzyme R160M, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
8.5 | - |
L-histidinol phosphate | mutant enzyme R197M, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
9.8 | - |
L-histidinol phosphate | mutant enzyme Y117A, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
18 | - |
L-histidinol phosphate | mutant enzyme E115Q, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
25 | - |
L-histidinol phosphate | mutant enzyme Y161A, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
36 | - |
L-histidinol phosphate | mutant enzyme HY157F, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
79 | - |
L-histidinol phosphate | mutant enzyme Y161F, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
89 | - |
L-histidinol phosphate | at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis | |
150 | - |
L-histidinol phosphate | mutant enzyme Y117F, at pH 9.0 and 22°C | Lactococcus lactis subsp. lactis |