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Literature summary for 3.1.3.12 extracted from
- Trehalose biosynthesis in Thermus thermophilus RQ-1: biochemical properties of the trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase (2005), Extremophiles, 9, 29-36.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ADP-glucose | activates 4.5% at 5 mM | Thermus thermophilus |  |
| GDP-glucose | activates 3.6% at 5 mM | Thermus thermophilus | |
| TDP-glucose | activates 4.6% at 5 mM | Thermus thermophilus | |
| UDP-glucose | activates 10% at 5 mM | Thermus thermophilus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene otsB, DNA and amino acid sequence determination and analysis, overexpression of GST-tagged enzyme in Eschrichia coli strains XL1-Blue and BL21 | Thermus thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Thermus thermophilus | |
| Ca2+ | - |
Thermus thermophilus | |
| Cu2+ | - |
Thermus thermophilus | |
| Sr2+ | - |
Thermus thermophilus | |
| Zn2+ | - |
Thermus thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
trehalose 6-phosphate | pH 7.0, 70°C, recombinant enzyme | Thermus thermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | stimulates | Thermus thermophilus | |
| Mg2+ | stimulates | Thermus thermophilus | |
| additional information | no effect by Mn2+, the enzyme requires divalent cations for maximal activity, in absence of metal ion the enzyme shows 32.5% of maximal activity | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trehalose 6-phosphate + H2O | Thermus thermophilus | the trehalose pathway involving trehalose-6-phosphate phosphatase plays a role in osmoadaptation, enzyme deficiency leads to reduced osmotolerance | trehalose + phosphate | - |
? | |
| trehalose 6-phosphate + H2O | Thermus thermophilus RQ-1 | the trehalose pathway involving trehalose-6-phosphate phosphatase plays a role in osmoadaptation, enzyme deficiency leads to reduced osmotolerance | trehalose + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | Q7WUI6 | gene otsB | - |
| Thermus thermophilus RQ-1 | Q7WUI6 | gene otsB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography, gel filtration, and ion exchange chromatography, to homogeneity, the GST-tag is cleaved off by thrombin | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + H2O | 3.1% of activity with trehalose 6-phosphate | Thermus thermophilus | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | 3.1% of activity with trehalose 6-phosphate | Thermus thermophilus RQ-1 | D-glucose + phosphate | - |
? | |
| additional information | glucose diphosphate nucleosides are no substrates | Thermus thermophilus | ? | - |
? | |
| additional information | glucose diphosphate nucleosides are no substrates | Thermus thermophilus RQ-1 | ? | - |
? | |
| trehalose 6-phosphate + H2O | the trehalose pathway involving trehalose-6-phosphate phosphatase plays a role in osmoadaptation, enzyme deficiency leads to reduced osmotolerance | Thermus thermophilus | trehalose + phosphate | - |
? | |
| trehalose 6-phosphate + H2O | high substrate specificity of recombinant enzyme | Thermus thermophilus | trehalose + phosphate | - |
? | |
| trehalose 6-phosphate + H2O | the trehalose pathway involving trehalose-6-phosphate phosphatase plays a role in osmoadaptation, enzyme deficiency leads to reduced osmotolerance | Thermus thermophilus RQ-1 | trehalose + phosphate | - |
? | |
| trehalose 6-phosphate + H2O | high substrate specificity of recombinant enzyme | Thermus thermophilus RQ-1 | trehalose + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TPP | - |
Thermus thermophilus |
| trehalose-6-phosphate phosphatase | - |
Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
recombinant enzyme | Thermus thermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 90 | recombinant enzyme, 45% of maximal activity at 90°C, about 15% at 40°C | Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
half-life of the recombinant enzyme is 161 min | Thermus thermophilus |
| 90 | - |
half-life of the recombinant enzyme is 120 min | Thermus thermophilus |
| 100 | - |
half-life of the recombinant enzyme is 40 min | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
about, recombinant enzyme | Thermus thermophilus |
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