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Literature summary for 3.1.3.11 extracted from
- Characterization and physiological role of two types of chloroplastic fructose-1,6-bisphosphatases in Euglena gracilis (2015), Arch. Biochem. Biophys., 575, 61-68 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain BL21 Star (DE3) pLysS | Euglena gracilis |
| gene EgFBPaseI, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR expression analysis, recombinant expression of His6-tagged isozyme | Euglena gracilis |
| gene EgFBPaseII, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR expression analysis, recombinant expression of His6-tagged isozyme | Euglena gracilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | EgFBPaseI gene suppression by by RNAi gene silencing | Euglena gracilis |
| additional information | EgFBPaseII gene suppression by by RNAi gene silencing | Euglena gracilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | isozyme EgFBPaseI is inhibited by 1 mM H2O2 and recovers when incubated with DTT | Euglena gracilis |  |
| additional information | isozyme EgFBPaseII is resistant to H2O2 up to concentrations of 1 mM; not inhibited by concentrations of AMP up to 1 mM; not inhibited by concentrations of AMP up to 1 mM | Euglena gracilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.165 | - |
D-fructose 1,6-bisphosphate | pH and temperature not specified in the publication | Euglena gracilis | |
| 0.165 | - |
D-fructose 1,6-bisphosphate | isozyme EgFBPaseI, pH 8.0, temperature not specified in the publication | Euglena gracilis | |
| 2.2 | - |
D-fructose 1,6-bisphosphate | pH and temperature not specified in the publication | Euglena gracilis | |
| 2.2 | - |
D-fructose 1,6-bisphosphate | isozyme EgFBPaseII, pH 8.0, temperature not specified in the publication | Euglena gracilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Euglena gracilis | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36900 | - |
EgFBPaseII fused with the hexahistidine-tag, SDS-PAGE | Euglena gracilis |
| 46300 | - |
EgFBPaseI fused with the hexahistidine-tag, SDS-PAGE | Euglena gracilis |
| 190000 | - |
about, isozyme EgFBPaseII, gel filtration | Euglena gracilis |
| 210000 | - |
about, isozyme EgFBPaseI, gel filtration | Euglena gracilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O | Euglena gracilis | - |
D-fructose 6-phosphate + phosphate | - |
? | |
| D-fructose 1,6-bisphosphate + H2O | Euglena gracilis Z | - |
D-fructose 6-phosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euglena gracilis | A0A0U5BQA3 | - |
- |
| Euglena gracilis | A3QSS7 | - |
- |
| Euglena gracilis Z | A0A0U5BQA3 | - |
- |
| Euglena gracilis Z | A3QSS7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Euglena gracilis |
| recombinant His6-tagged isozyme by nickel affinity chromatography | Euglena gracilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O | - |
Euglena gracilis | D-fructose 6-phosphate + phosphate | - |
? | |
| D-fructose 1,6-bisphosphate + H2O | - |
Euglena gracilis Z | D-fructose 6-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 36900, recombinant His6-tagged isozyme EgFBPaseII, SDS-PAGE | Euglena gracilis |
| homotetramer | 4 * 46300, recombinant His6-tagged isozyme EgFBPaseI, SDS-PAGE | Euglena gracilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EgFBPaseI | - |
Euglena gracilis |
| EgFBPaseII | - |
Euglena gracilis |
| FBPase | - |
Euglena gracilis |
| fructose-1,6-bisphosphatase | - |
Euglena gracilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
D-fructose 1,6-bisphosphate | pH and temperature not specified in the publication | Euglena gracilis | |
| 0.17 | - |
D-fructose 1,6-bisphosphate | isozyme EgFBPaseI, pH 8.0, temperature not specified in the publication | Euglena gracilis | |
| 1.08 | - |
D-fructose 1,6-bisphosphate | pH and temperature not specified in the publication | Euglena gracilis | |
| 1.08 | - |
D-fructose 1,6-bisphosphate | isozyme EgFBPaseII, pH 8.0, temperature not specified in the publication | Euglena gracilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Euglena gracilis |
| 9 | - |
- |
Euglena gracilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.5 | pH 8.0: about 40% of maximal activity, pH 9-5: about 70% of maximal activity | Euglena gracilis |
| 8 | 9.5 | pH 8.0: about 60% of maximal activity, pH 9.5: about 70% of maximal activity | Euglena gracilis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | chloroplastic FBPase isoenzymes are widely distributed in photosynthetic organisms, i.e. bacteria, blue-green and green algae, lichens, and plants | Euglena gracilis |
| malfunction | EgFBPaseI gene suppression by gene silencing markedly decreases photosynthetic activity and inhibits cell growth | Euglena gracilis |
| metabolism | the chloroplastic fructose-1,6-bisphosphatase (FBPase) is a late-limiting enzyme in the Calvin cycle | Euglena gracilis |
| metabolism | the enzyme (EgFBPaseI) is critical for the Calvin cycle in Euglena chloroplasts in order to maintain normal cell growth under photoautotrophic conditions | Euglena gracilis |
| physiological function | fructose-1,6-bisphophatase catalyzes the breakdown of fructose 1,6-bisphosphate to fructose 6-phosphate and phosphate. Two FBPase isoenzymes are identified in photosynthetic eukaryotic cells: a cytosolic form, a key enzyme in gluconeogenesis, and a chloroplastic form, a rate-limiting enzyme involved in the Calvin cycle | Euglena gracilis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.49 | - |
D-fructose 1,6-bisphosphate | isozyme EgFBPaseII, pH 8.0, temperature not specified in the publication | Euglena gracilis | |
| 0.5 | - |
D-fructose 1,6-bisphosphate | pH and temperature not specified in the publication | Euglena gracilis | |
| 1 | - |
D-fructose 1,6-bisphosphate | pH and temperature not specified in the publication | Euglena gracilis | |
| 1.03 | - |
D-fructose 1,6-bisphosphate | isozyme EgFBPaseI, pH 8.0, temperature not specified in the publication | Euglena gracilis | |
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