Literature summary for 3.1.3.11 extracted from

Goswami, R.; Bondoc, J.M.G.; Wheeler, P.R.; Jafari, A.; Gonzalez, T.; Mehboob, S.; Movahedzadeh, F.
Inositol monophosphatase A bifunctional enzyme in Mycobacterium smegmatis (2018), ACS Omega, 3, 13876-13881 .

Search for more literature for 3.1.3.11

Cloned(Commentary)

Cloned (Comment)	Organism
-	Mycolicibacterium smegmatis

Protein Variants

Protein Variants	Comment	Organism
D221A	loss of 60-70% of activity for both FBPase and IMPase activities	Mycolicibacterium smegmatis
G94A/T95A	mutations partially restore the IMPase activity compared to the single mutants	Mycolicibacterium smegmatis
T95A	about 60% loss of both FBPase and IMPase activities	Mycolicibacterium smegmatis
T95S	about 35% loss of both FBPase and IMPase activities	Mycolicibacterium smegmatis
W220A	loss of 60-70% of activity for both FBPase and IMPase activities	Mycolicibacterium smegmatis
W220A/D221A	mutations reduces both FBPase and IMPase activity drastically	Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0111	-	D-fructose 1,6-diphosphate	pH 8.0, 23°C	Mycolicibacterium smegmatis

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium smegmatis	A0QX86	bifunctional enzyme,also functions as a inositol monphosphatase, EC 3.1.3.25	-
Mycolicibacterium smegmatis ATCC 700084	A0QX86	bifunctional enzyme,also functions as a inositol monphosphatase, EC 3.1.3.25	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-diphosphate + H2O	-	Mycolicibacterium smegmatis	D-fructose 6-phosphate + phosphate	-	?
D-fructose 1,6-diphosphate + H2O	-	Mycolicibacterium smegmatis ATCC 700084	D-fructose 6-phosphate + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
ImpA	-	Mycolicibacterium smegmatis
IMPase A	-	Mycolicibacterium smegmatis

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Release 2023.1 (January 2023)