Literature summary for 3.1.3.11 extracted from

Liang, P.; Sun, J.; Huang, Y.; Zhang, F.; Zhou, J.; Hu, Y.; Wang, X.; Liang, C.; Zheng, M.; Xu, Y.; Mao, Q.; Hu, X.; Li, X.; Xu, J.; Lu, G.; Yu, X.
Biochemical characterization and functional analysis of fructose-1,6-bisphosphatase from Clonorchis sinensis (2013), Mol. Biol. Rep., 40, 4371-4382.

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Application

Application	Comment	Organism
medicine	recombinant enzyme can specifically bind to the membrane of human hepatic stellate cell line LX-2 and stimulates proliferation and activation of LX-2, provoking upregulation of key fibrosis-related factors, such as alpha-smooth muscle actin, collagen I and collagen III. In infected rat, the enzyme is ubiquitously detected on the bile duct epithelial cells and the surface of hyperplastic adenoma close to the resident worms and the liver parenchyma, respectively	Clonorchis sinensis

Inhibitors

Inhibitors	Comment	Organism	Structure
AMP	inhibitory below 0.2 mM	Clonorchis sinensis
Ca2+	unique divalent metal ion inhibitor	Clonorchis sinensis
Mg2+	inhibitory above 10 mM	Clonorchis sinensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0419	-	D-fructose 1,6-bisphosphate	pH 7.5, 37°C	Clonorchis sinensis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Clonorchis sinensis	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	stimulation increases in the order Mg2+, Mn2+, Zn2+, Cu2+. Mg2+ stimulates below 5 mM, inhibits above 10 mM	Clonorchis sinensis
Mg2+	stimulation increases in the order Mg2+, Mn2+, Zn2+, Cu2+. Mg2+ stimulates below 5 mM, inhibits above 10 mM	Clonorchis sinensis
Mn2+	stimulation increases in the order Mg2+, Mn2+, Zn2+, Cu2+. Mg2+ stimulates below 5 mM, inhibits above 10 mM	Clonorchis sinensis
Zn2+	stimulation increases in the order Mg2+, Mn2+, Zn2+, Cu2+. Mg2+ stimulates below 5 mM, inhibits above 10 mM	Clonorchis sinensis

Organism

Organism	UniProt	Comment	Textmining
Clonorchis sinensis	G7YVB4	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
intestine	-	Clonorchis sinensis	-
larval integument	-	Clonorchis sinensis	-
additional information	both transcriptional and translational level are higher in metacercariae of larva stages, than those in adult worm. Ubiquitous expression in metacercariae	Clonorchis sinensis	-
vitellarium	-	Clonorchis sinensis	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-bisphosphate + H2O	-	Clonorchis sinensis	D-fructose 6-phosphate + phosphate	-	?

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
15	45	more than 70% of maxiumum activity	Clonorchis sinensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	8	-	Clonorchis sinensis

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	9.5	more than 80% of maxiumum activity	Clonorchis sinensis

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Release 2023.1 (January 2023)