Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, sitting drop vapour diffusion method, 20°C, X-ray diffraction structure determination and analysis of twinned crystals at 1.2 A resolution, and of untwinned crystals at 1.8 A resolution | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40324 | - |
2 * 40324, mass spectrometry, 2 * 40361, sequence calculation | Thermus thermophilus |
40361 | - |
2 * 40324, mass spectrometry, 2 * 40361, sequence calculation | Thermus thermophilus |
80000 | - |
gel filtration | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | Thermus thermophilus | - |
D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
D-fructose 6-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q5SJM8 | ATCC 27634 | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SJM8 | ATCC 27634 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by heat treatment, nickel affinity chromatography, and gel filtration | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | - |
Thermus thermophilus | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | D-fructose 6-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40324, mass spectrometry, 2 * 40361, sequence calculation | Thermus thermophilus |
More | in solution, the enzyme appears to be a homodimer which ensembles into an octamer in the tetragonal crystals | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
archaeal-like fructose-1,6-bisphosphatase | - |
Thermus thermophilus |
FBPase | - |
Thermus thermophilus |
fructose-1,6-bisphosphatase | - |
Thermus thermophilus |