Literature summary for 3.1.3.11 extracted from

Gutka, H.J.; Franzblau, S.G.; Movahedzadeh, F.; Abad-Zapatero, C.
Crystallization and preliminary X-ray characterization of the glpX-encoded class II fructose-1,6-bisphosphatase from Mycobacterium tuberculosis (2011), Acta Crystallogr. Sect. F, 67, 710-713.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3)	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged enzyme, hanging-drop vapor-diffusion method, 0.001 ml of protein solution containing 10 mg/ml protein, is mixed with 0.001 ml of reservoir solution containing 1.8 M ammonium citrate tribasic, pH 7.0, and equilibratzion against 0.3 ml reservoir solution, 72 h to 2 weeks, X-ray diffraction structure determination and analysis at 2.7 A resolution, molecular replacement	Mycobacterium tuberculosis

Crystallization (Comment)

Organism

purified recombinant His-tagged enzyme, hanging-drop vapor-diffusion method, 0.001 ml of protein solution containing 10 mg/ml protein, is mixed with 0.001 ml of reservoir solution containing 1.8 M ammonium citrate tribasic, pH 7.0, and equilibratzion against 0.3 ml reservoir solution, 72 h to 2 weeks, X-ray diffraction structure determination and analysis at 2.7 A resolution, molecular replacement

Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37000	-	x * 37000, recombinant His-tagged enzyme, SDS-PAGE	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-fructose 1,6-bisphosphate + H2O	Mycobacterium tuberculosis	-	D-fructose 6-phosphate + phosphate	-	?
D-fructose 1,6-bisphosphate + H2O	Mycobacterium tuberculosis H37Rv	-	D-fructose 6-phosphate + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	gene glpX	-
Mycobacterium tuberculosis H37Rv	-	gene glpX	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-bisphosphate + H2O	-	Mycobacterium tuberculosis	D-fructose 6-phosphate + phosphate	-	?
D-fructose 1,6-bisphosphate + H2O	-	Mycobacterium tuberculosis H37Rv	D-fructose 6-phosphate + phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 37000, recombinant His-tagged enzyme, SDS-PAGE	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
class II FBPase	-	Mycobacterium tuberculosis
class II fructose-1,6-bisphosphatase	-	Mycobacterium tuberculosis
FBPase	-	Mycobacterium tuberculosis

General Information

General Information	Comment	Organism
metabolism	fructose-1,6-bisphosphatase is a key enzyme in gluconeogenesis	Mycobacterium tuberculosis