Literature summary for 3.1.3.11 extracted from

Rakus, D.; Maciaszczyk, E.; Wawrzycka, D.; Ulaszewski, S.; Eschrich, K.; Dzugaj, A.
The origin of the high sensitivity of muscle fructose 1,6-bisphosphatase towards AMP (2005), FEBS Lett., 579, 5577-5581.

Search for more literature for 3.1.3.11

Protein Variants

Protein Variants	Comment	Organism
E20K	liver enzyme, mutation towards the muscle enzyme	Homo sapiens
E20K/M177T/C179Q	liver enzyme, mutation towards the muscle enzyme	Homo sapiens
K20E	muscle enzyme, mutation towards the liver enzyme	Homo sapiens
K20E/T177M/Q179C	muscle enzyme, mutation towards the liver enzyme	Homo sapiens
M177T/C179Q	liver enzyme, mutation towards the muscle enzyme	Homo sapiens
T177M/Q179C	muscle enzyme, mutation towards the liver enzyme	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-monophosphate	wild-type muscle enzyme, 50% inhibition at 0.00022 mM, wild-type liver enzyme, 50% inhibition at 0.0046 mM	Homo sapiens
AMP	wild-type muscle enzyme, 50% inhibition at 0.0001 mM, Hill coefficient 1.78, wild-type liver enzyme, 50% inhibition at 0.0044 mM, Hill coefficient 2.08	Homo sapiens
D-fructose 1,6-bisphosphate	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0013	-	D-fructose 1,6-bisphosphate	wild-type muscle enzyme, pH 7.5, 37°C	Homo sapiens
0.0015	-	D-fructose 1,6-bisphosphate	liver enzyme mutant E20K/M177T/C179Q, pH 7.5, 37°C	Homo sapiens
0.0015	-	D-fructose 1,6-bisphosphate	muscle enzyme mutant T177M/Q179C, pH 7.5, 37°C	Homo sapiens
0.0017	-	D-fructose 1,6-bisphosphate	muscle enzyme mutant K20E/T177M/Q179C, pH 7.5, 37°C	Homo sapiens
0.0019	-	D-fructose 1,6-bisphosphate	liver enzyme mutant M177T/C179Q, pH 7.5, 37°C	Homo sapiens
0.0019	-	D-fructose 1,6-bisphosphate	muscle enzyme mutant K20E, pH 7.5, 37°C	Homo sapiens
0.0021	-	D-fructose 1,6-bisphosphate	wild-type liver enzyme, pH 7.5, 37°C	Homo sapiens
0.0023	-	D-fructose 1,6-bisphosphate	liver enzyme mutant E20K, pH 7.5, 37°C	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	wild-type muscle enzyme, Hill coefficient 1.89, wild-type liver enzyme, hill coefficient 1.83	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P09467	liver and muscle isoforms	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Homo sapiens	-
muscle	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-bisphosphate + H2O	-	Homo sapiens	D-fructose 6-phosphate + phosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
17.6	-	D-fructose 1,6-bisphosphate	muscle enzyme mutant K20E/T177M/Q179C, pH 7.5, 37°C	Homo sapiens
18.4	-	D-fructose 1,6-bisphosphate	liver enzyme mutant M177T/C179Q, pH 7.5, 37°C	Homo sapiens
18.6	-	D-fructose 1,6-bisphosphate	liver enzyme mutant E20K, pH 7.5, 37°C	Homo sapiens
19.9	-	D-fructose 1,6-bisphosphate	muscle enzyme mutant T177M/Q179C, pH 7.5, 37°C	Homo sapiens
20.8	-	D-fructose 1,6-bisphosphate	wild-type liver enzyme, pH 7.5, 37°C	Homo sapiens
21.1	-	D-fructose 1,6-bisphosphate	muscle enzyme mutant K20E, pH 7.5, 37°C	Homo sapiens
21.7	-	D-fructose 1,6-bisphosphate	liver enzyme mutant E20K/M177T/C179Q, pH 7.5, 37°C	Homo sapiens
23.4	-	D-fructose 1,6-bisphosphate	wild-type muscle enzyme, pH 7.5, 37°C	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00013	-	D-fructose 1,6-bisphosphate	muscle enzyme mutant T177M/Q179C, pH 7.5, 37°C	Homo sapiens
0.00016	-	D-fructose 1,6-bisphosphate	muscle enzyme mutant K20E/T177M/Q179C, pH 7.5, 37°C	Homo sapiens
0.00016	-	D-fructose 1,6-bisphosphate	wild-type muscle enzyme, pH 7.5, 37°C	Homo sapiens
0.00017	-	D-fructose 1,6-bisphosphate	muscle enzyme mutant K20E, pH 7.5, 37°C	Homo sapiens
0.00018	-	D-fructose 1,6-bisphosphate	liver enzyme mutant E20K/M177T/C179Q, pH 7.5, 37°C	Homo sapiens
0.00019	-	D-fructose 1,6-bisphosphate	liver enzyme mutant M177T/C179Q, pH 7.5, 37°C	Homo sapiens
0.00023	-	D-fructose 1,6-bisphosphate	wild-type liver enzyme, pH 7.5, 37°C	Homo sapiens
0.00024	-	D-fructose 1,6-bisphosphate	liver enzyme mutant E20K, pH 7.5, 37°C	Homo sapiens

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Release 2023.1 (January 2023)