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Literature summary for 3.1.3.11 extracted from
- Purification and characterization of a recombinant pea cytoplasmic fructose-1,6-bisphosphatase (2003), Protein Expr. Purif., 28, 42-48.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pisum sativum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | non-competitive inhibition | Pisum sativum |  |
| D-fructose-2,6-bisphosphate | competitive inhibition | Pisum sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0025 | - |
D-fructose 1,6-bisphosphate | pH 6.6, 25°C, native enzyme | Pisum sativum | |
| 0.005 | - |
D-fructose 1,6-bisphosphate | pH 6.6, 25°C, Trombin treated enzyme | Pisum sativum | |
| 0.01 | - |
D-fructose 1,6-bisphosphate | pH 6.6, 25°C, recombinant enzyme | Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Pisum sativum | 9507 | - |
| cytoplasm | - |
Pisum sativum | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
- |
Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O | Pisum sativum | the the chloroplastic isozyme plays a key role in the Calvin cycle and the cytoplasmic isozyme plays a key role in the sucrose synthesis in cytoplasm | D-fructose 6-phosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Pisum sativum | Q8RW99 | - |
- |
| Spinacia oleracea | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| the recombinant enzyme | Pisum sativum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.16 | - |
- |
Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O | - |
Pisum sativum | D-fructose 6-phosphate + phosphate | - |
? | |
| D-fructose 1,6-bisphosphate + H2O | the the chloroplastic isozyme plays a key role in the Calvin cycle and the cytoplasmic isozyme plays a key role in the sucrose synthesis in cytoplasm | Pisum sativum | D-fructose 6-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 38000, SDS-PAGE | Pisum sativum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
optimum of recombinant enzyme | Pisum sativum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
the native enzyme is completely inactivated at temperatures higher than 45°C | Pisum sativum |
| 55 | - |
the activity of recombinant enzyme is increased at temperatures higher than 55°C | Pisum sativum |
| 65 | - |
the recombinant enzyme is completely inactivated at temperatures higher than 65°C | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
the recombinant enzyme | Pisum sativum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | the recombinant enzyme | Pisum sativum |
| 7.5 | 8.2 | the native enzyme | Pisum sativum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00007 | - |
D-fructose 2,6-bisphosphate | pH 6.6, 25°C, native enzyme | Pisum sativum | |
| 0.0001 | - |
D-fructose 2,6-bisphosphate | pH 6.6, 25°C, Trombin treated enzyme | Pisum sativum | |
| 0.00015 | - |
D-fructose 2,6-bisphosphate | pH 6.6, 25°C, recombinant enzyme | Pisum sativum | |
| 0.12 | - |
AMP | pH 6.6, 25°C, native enzyme | Pisum sativum | |
| 0.16 | - |
AMP | pH 6.6, 25°C, recombinant enzyme | Pisum sativum | |
| 0.16 | - |
AMP | pH 6.6, 25°C, Trombin treated enzyme | Pisum sativum | |
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