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Literature summary for 3.1.3.11 extracted from
- Overexpression in Escherichia coli and characterization of the chloroplast fructose-1,6-bisphosphatase from wheat (2000), Protein Expr. Purif., 19, 411-418.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| guanidine hydrochloride | at low concentrations | Triticum aestivum |  |
| Urea | at concentrations lower than 0.8 M | Triticum aestivum | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Triticum aestivum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | at high concentration depending on the concentration of Mg2+ | Triticum aestivum | |
| K+ | - |
Triticum aestivum | |
| NH4+ | - |
Triticum aestivum | |
| Zn2+ | the enzyme activity is completely inhibited at concentrations higher than 1 mM in presence of 10 mM Mg2+ | Triticum aestivum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.121 | - |
D-fructose 1,6-bisphosphate | pH 8.8, 25°C, recombinant enzyme | Triticum aestivum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Triticum aestivum | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | at low concentrations | Triticum aestivum | |
| dithiothreitol | the recombinant enzyme is activated | Triticum aestivum | |
| Li+ | 0.05-0.2 M, 20% activation in presence of 10 mM Mg2+ | Triticum aestivum | |
| Mg2+ | required | Triticum aestivum | |
| Na+ | 0.05-0.5 M, 20% activation in presence of 10 mM Mg2+ | Triticum aestivum | |
| Zn2+ | at low concentrations | Triticum aestivum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38900 | - |
recombinant enzyme | Triticum aestivum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O | Triticum aestivum | the enzyme plays a key role in the Calvin cycle | D-fructose 6-phosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| the recombinant enzyme | Triticum aestivum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
the recombinant enzyme | Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O | - |
Triticum aestivum | D-fructose 6-phosphate + phosphate | - |
? | |
| D-fructose 1,6-bisphosphate + H2O | the enzyme plays a key role in the Calvin cycle | Triticum aestivum | D-fructose 6-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 38900, SDS-PAGE, recombinant enzyme | Triticum aestivum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
50 | the recombinant enzyme is stable | Triticum aestivum |
| 60 | - |
10% loss of activity | Triticum aestivum |
| 70 | - |
40% loss of activity | Triticum aestivum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 22.9 | - |
D-fructose 1,6-bisphosphate | pH 8.8, 25°C, recombinant enzyme | Triticum aestivum | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
Ca2+ | pH 8.8, 25°C, the recombinant enzyme in presence of 2 mM Mg2+ | Triticum aestivum | |
| 2.5 | - |
Ca2+ | pH 8.8, 25°C, the recombinant enzyme in presence of 10 mM Mg2+ | Triticum aestivum | |
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