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Literature summary for 3.1.3.11 extracted from

  • Reichert, A.; Baalmann, E.; Vetter, S.; Backhausen, J.E.; Scheibe, R.
    Activation properties of the redox-modulated chloroplast enzymes glyceraldehyde 3-phosphate dehydrogenase and fructose-1,6-bisphosphatase (2000), Physiol. Plant., 110, 330-341.
No PubMed abstract available
Search for more literature for 3.1.3.11

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ in presence of fructose-1,6-bisphosphate, activates the native enzyme and recombinant FBPase4.5, not FBPase4.9 Spinacia oleracea
thioredoxin
-
 Spinacia oleracea

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Spinacia oleracea 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ comparison of activity at 2, 10 and 15 mM Mg2+ of native and two recombinant enzymes Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 1,6-bisphosphate + H2O Spinacia oleracea
-
 D-fructose 6-phosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Spinacia oleracea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate + H2O
-
 Spinacia oleracea D-fructose 6-phosphate + phosphate
-
 ?

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 comparison of activity at pH 8.0 and 8.8 of native and two recombinant enzymes Spinacia oleracea

pI Value

Organism Comment pI Value Maximum pI Value
Spinacia oleracea FBPase4.5, the recombinant enzyme in oxidized and reduced state has a pI value of 4.5, isoelectric focusing
-
 4.5
Spinacia oleracea FBPase4.9, the recombinant enzyme in oxidized state has a pI value of 4.9, after reduction with DTT the pI value changes to 4.5 and remains at 4.5 even after re-oxidation, isoelectric focusing
-
 4.9