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Literature summary for 3.1.3.11 extracted from
- Purification and characterization of glpX-encoded fructose 1, 6-bisphosphatase, a new enzyme of the glycerol 3-phosphate regulon of Escherichia coli (2000), J. Bacteriol., 182, 5624-5627.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphoenolpyruvate | - |
Escherichia coli |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Escherichia coli | |
| ATP | - |
Escherichia coli | |
| D-fructose 1,6-bisphosphate | substrate inhibition at high concentrations | Escherichia coli | |
| D-Fructose 1-phosphate | - |
Escherichia coli | |
| additional information | not: AMP | Escherichia coli | |
| phosphate | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
D-fructose-1,6-bisphosphate | pH 7.7 | Escherichia coli | |
| 1 | - |
D-Fructose 1-phosphate | pH 7.7 | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli | |
| Mn2+ | the omission of 2 mM Mn2+ in assay results in 90% loss of activity | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
2 * 40000, SDS-PAGE | Escherichia coli |
| 80000 | 90000 | gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O | Escherichia coli | enzyme functions with FBPase I in the centarle pathways of carbohydrate metabolism | D-fructose 6-phosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0A9C9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O | best substrate | Escherichia coli | D-fructose 6-phosphate + phosphate | - |
? | |
| D-fructose 1,6-bisphosphate + H2O | enzyme functions with FBPase I in the centarle pathways of carbohydrate metabolism | Escherichia coli | D-fructose 6-phosphate + phosphate | - |
? | |
| D-fructose 1-phosphate + H2O | - |
Escherichia coli | D-fructose + phosphate | - |
? | |
| ribulose 1,5-bisphosphate + H2O | 15% of activity obtained with D-fructose 1,6-bisphosphate | Escherichia coli | ribulose 5-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 40000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FBPase II | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
phosphate | - |
Escherichia coli | |
| 1 | - |
D-Fructose 1-phosphate | pH 7.7 | Escherichia coli | |
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