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Literature summary for 3.1.3.11 extracted from
- Purification and properties of a D-fructose 1,6-bisphosphatase from Saccharomyces cerevisiae (1979), Arch. Biochem. Biophys., 197, 170-177.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-deoxy-AMP | - |
Saccharomyces cerevisiae |  |
| AMP | - |
Saccharomyces cerevisiae | |
| fructose 1,6-diphosphate | no inhibition up to 1 mM | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
fructose 1,6-diphosphate | at 10 mM Mg2+ | Saccharomyces cerevisiae | |
| 0.01 | - |
fructose 1,6-diphosphate | at 2 mM Mg2+ | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
2 * 56000, SDS-PAGE | Saccharomyces cerevisiae |
| 115000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate | uni-bi mechanism | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 44.6 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-diphosphate + H2O | - |
Saccharomyces cerevisiae | D-fructose 6-phosphate + phosphate | - |
? | |
| glucose 1,6-diphosphate + H2O | about 10% of maximal activity | Saccharomyces cerevisiae | ? | - |
? | |
| sedoheptulose 1,7-bisphosphate + H2O | about 5% of maximal activity | Saccharomyces cerevisiae | sedoheptulose 7-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 56000, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | pH 6.0: about 25% of maximal activity, pH 11.0.: 55% of maximal activity | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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