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Literature summary for 3.1.3.11 extracted from

  • Amachi, T.; Bowien, B.
    Characterization of two fructose bisphosphatase isoenzymes from the hydrogen bacterium Nocardia opaca 1b (1979), J. Gen. Microbiol., 113, 347-356.
No PubMed abstract available
Search for more literature for 3.1.3.11

Activating Compound

Activating Compound Comment Organism Structure
EDTA stimulates Rhodococcus opacus

Inhibitors

Inhibitors Comment Organism Structure
AMP 0.1 mM, 9% inhibition of isoenzyme A, complete inhibition of isoenzyme B Rhodococcus opacus
ATP
-
 Rhodococcus opacus
Mn2+ inhibition above 0.5 mM Rhodococcus opacus
NAD+ no inhibition of isoenzyme A, 67% inhibition of isoenzyme B Rhodococcus opacus
NADH 20% inhibition of isoenzyme A, 83% inhibition of isoenzyme B Rhodococcus opacus
Ribulose 1,5-diphosphate 1 mM, 48% inhibition of isoenzyme A, 3% inhibition of isoenzyme B Rhodococcus opacus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 sedoheptulose 1,7-diphosphate isoenzyme A and B Rhodococcus opacus
0.017
-
 fructose 1,6-diphosphate isoenzyme A and B Rhodococcus opacus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Rhodococcus opacus
Mn2+ can partially replace Mg2+ in activation Rhodococcus opacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 isoenzyme A, gel filtration Rhodococcus opacus
190000
-
 isoenzyme B, gel filtration Rhodococcus opacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 1,6-diphosphate + H2O Rhodococcus opacus isoenzyme A is present when the organism is grown under autotrophic conditions but is not synthesized during heterotrophic growth. Isoenzyme B is detected in both autothrophically and heterotrophically grown organisms ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus opacus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, 2 isoenzymes: A and B Rhodococcus opacus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.75
-
 isoenzyme B, hydrolysis of sedoheptulose 1,6-diphosphate Rhodococcus opacus
3.39
-
 isoenzyme B, hydrolysis of fructose 1,6-diphosphate Rhodococcus opacus
11.82
-
 isoenzyme A, hydrolysis of fructose 1,6-diphosphate Rhodococcus opacus
20.45
-
 isoenzyme B, hydrolysis of sedoheptulose 1,6-diphosphate Rhodococcus opacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-diphosphate + H2O isoenzyme A is present when the organism is grown under autotrophic conditions but is not synthesized during heterotrophic growth. Isoenzyme B is detected in both autothrophically and heterotrophically grown organisms Rhodococcus opacus ?
-
 ?
D-fructose 1,6-diphosphate + H2O
-
 Rhodococcus opacus D-fructose 6-phosphate + phosphate
-
 ?
sedoheptulose 1,7-bisphosphate + H2O
-
 Rhodococcus opacus sedoheptulose 7-phosphate + phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 isoenzyme A, hydrolysis of fructose 1,6-diphosphate or sedoheptulose 1,7-diphosphate Rhodococcus opacus
8.8 9 isoenzyme B, hydrolysis of fructose 1,6-diphosphate or sedoheptulose 1,7-diphosphate Rhodococcus opacus