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Literature summary for 3.1.3.100 extracted from
- Copurification of chicken liver soluble thiamine monophosphatase and low molecular weight acid phosphatase (2017), Ukr. Biochem. J., 89, 13-21 .
No PubMed abstract available
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Gallus gallus | 5829 | - |
| soluble | - |
Gallus gallus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Mg2+, Mn2+ or Ca2+ do not affect the enzyme activity | Gallus gallus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17800 | - |
gel filtration | Gallus gallus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiamine phosphate + H2O | Gallus gallus | - |
thiamine + phosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Ni-NTA column chromatography, and Servacel P-23 chromatography, and Sephadex G-50 gel filtration | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Gallus gallus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | 100% activity | Gallus gallus | 4-nitrophenol + phosphate | - |
? | |
| FMN + H2O | 73% activity compared to 4-nitrophenyl phosphate | Gallus gallus | ? | - |
? | |
| L-phosphotyrosine + H2O | 32% activity compared to 4-nitrophenyl phosphate | Gallus gallus | phosphate + L-tyrosine | - |
? | |
| additional information | less than 1% activity with pyridoxal 5'-phosphate, beta-glycerophosphate, D-glucose 6-phosphate, D-fructose 1,6-diphosphate, inosine 5'-monophosphate, or adenosine 5'-monophosphate | Gallus gallus | ? | - |
? | |
| thiamine phosphate + H2O | - |
Gallus gallus | thiamine + phosphate | - |
? | |
| thiamine phosphate + H2O | 18% activity compared to 4-nitrophenyl phosphate | Gallus gallus | thiamine + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| thiamine monophosphatase | - |
Gallus gallus |
| ThMPase | - |
Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Gallus gallus |
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Release 2023.1 (January 2023)
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