Literature summary for 3.1.3.1 extracted from
Homaei, A.
Purification and biochemical properties of highly efficient alkaline phosphatase from Fenneropenaeus merguiensis brain (2015), J. Mol. Catal. B, 118, 16-22 .
No PubMed abstract available
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
Ca2+ |
83% residual activity at 5 mM |
Penaeus merguiensis |
|
Cu2+ |
complete inhibition at 2 mM |
Penaeus merguiensis |
|
Hg2+ |
complete inhibition at 2 mM |
Penaeus merguiensis |
|
K+ |
90% residual activity at 4 mM |
Penaeus merguiensis |
|
Li+ |
83% residual activity at 5 mM |
Penaeus merguiensis |
|
Mg2+ |
93% residual activity at 5 mM |
Penaeus merguiensis |
|
Na+ |
91% residual activity at 5 mM |
Penaeus merguiensis |
|
Pb2+ |
complete inhibition at 2 mM |
Penaeus merguiensis |
|
KM Value [mM]
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
0.0003 |
- |
4-nitrophenyl phosphate |
at pH 11.0 and 25°C |
Penaeus merguiensis |
|
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
Ca2+ |
125% activity at 2 mM |
Penaeus merguiensis |
|
Mg2+ |
123% activity at 1 mM |
Penaeus merguiensis |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Penaeus merguiensis |
- |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
ammonium sulfate precipitation, DEAE-cellulose column chromatography, DEAE-Sephadex gel filtration, and Sephadex G-200 gel filtration |
Penaeus merguiensis |
Source Tissue
Source Tissue |
Comment |
Organism |
Textmining |
brain |
- |
Penaeus merguiensis |
- |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
4-nitrophenyl phosphate + H2O |
strongest affinity substrate |
Penaeus merguiensis |
4-nitrophenol + phosphate |
- |
? |
|
Subunits
Subunits |
Comment |
Organism |
? |
x * 70000, SDS-PAGE |
Penaeus merguiensis |
Synonyms
Synonyms |
Comment |
Organism |
ALPase |
- |
Penaeus merguiensis |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
50 |
- |
- |
Penaeus merguiensis |
Temperature Range [°C]
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
30 |
70 |
the enzyme retains a high activity (more than 50%) in a wide temperature range from about 30-70°C. About 35% of its maximum phosphatase activity is retained at low (25°C) and very high (80°C) temperatures |
Penaeus merguiensis |
Turnover Number [1/s]
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
95 |
- |
4-nitrophenyl phosphate |
at pH 11.0 and 25°C |
Penaeus merguiensis |
|
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
11 |
- |
- |
Penaeus merguiensis |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
6 |
12 |
the enzyme retains a high activity (more than 50%) in the pH range from pH 6.0-12.0 |
Penaeus merguiensis |
kcat/KM [mM/s]
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Comment |
Organism |
Structure |
316600 |
- |
4-nitrophenyl phosphate |
at pH 11.0 and 25°C |
Penaeus merguiensis |
|