Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.1 extracted from

  • Bhardwaj, R.; Skelly, P.J.
    Characterization of schistosome tegumental alkaline phosphatase (SmAP) (2011), PLoS Negl. Trop. Dis., 5, e1011.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
predicted from cDNA, Western blot Schistosoma mansoni
120000
-
Western blot, in the absence of reducing agent Schistosoma mansoni

Organism

Organism UniProt Comment Textmining
Schistosoma mansoni A8TKU6
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Schistosoma mansoni

Source Tissue

Source Tissue Comment Organism Textmining
additional information SmAP gene is most highly expressed in intravascular parasite life stages. Using immunofluorescence and immuno-electron microscopy, it is shown that SmAP is expressed at the host/parasite interface and in internal tissues Schistosoma mansoni
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Schistosoma mansoni 4-nitrophenol + phosphate
-
?
AMP + H2O
-
Schistosoma mansoni adenosine + phosphate
-
?

Subunits

Subunits Comment Organism
homodimer Western blot analysis, 2 * 60000 Schistosoma mansoni

Synonyms

Synonyms Comment Organism
SmAP
-
Schistosoma mansoni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Schistosoma mansoni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.9
-
assay at Schistosoma mansoni

General Information

General Information Comment Organism
malfunction the ability of living parasites to cleave exogenous adenosine monophosphate and generate adenosine is very largely abolished when SmAP gene expression is suppressed following RNAi treatment targeting the gene Schistosoma mansoni