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Literature summary for 3.1.3.1 extracted from
- Purple acid phosphatase-like sequences in prokaryotic genomes and the characterization of an atypical purple alkaline phosphatase from Burkholderia cenocepacia J2315 (2009), Gene, 440, 1-8.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| GST-fusion protein expressed in Escherichia coli BL21 | Burkholderia cenocepacia |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D199A | metal-ligating residue, no activity at pH 8.5 | Burkholderia cenocepacia |
| H237A | non-metal linking residue, no activity at pH 8.5 | Burkholderia cenocepacia |
| Y202A | metal-ligating residue, no activity at pH 8.5 | Burkholderia cenocepacia |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Burkholderia cenocepacia |  |
| Ni2+ | - |
Burkholderia cenocepacia | |
| Sodium molybdate | - |
Burkholderia cenocepacia | |
| Zn2+ | - |
Burkholderia cenocepacia | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.145 | - |
phosphoenolpyruvate | - |
Burkholderia cenocepacia | |
| 0.33 | - |
p-nitrophenyl phosphate | - |
Burkholderia cenocepacia | |
| 0.806 | - |
O-Phosphotyrosine | - |
Burkholderia cenocepacia | |
| 1.096 | - |
O-phosphoserine | - |
Burkholderia cenocepacia | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular space | - |
Burkholderia cenocepacia | 5615 | - |
| periplasmic space | - |
Burkholderia cenocepacia | 42597 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | Fe(III) and Zn(II) binuclear centre | Burkholderia cenocepacia | |
| Zn2+ | Fe(III) and Zn(II) binuclear centre | Burkholderia cenocepacia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
1 * 57000, SDS-PAGE, gel filtration | Burkholderia cenocepacia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cenocepacia | B4EKR2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography | Burkholderia cenocepacia |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.02 | - |
substrate DLDVPIPGRDFRRVpSVAAE | Burkholderia cenocepacia |
| 1.1 | - |
substrate ENCpYINASL | Burkholderia cenocepacia |
| 2.52 | - |
substrate phosphoenolpyruvate | Burkholderia cenocepacia |
| 2.59 | - |
substrate phosphoserine | Burkholderia cenocepacia |
| 13.73 | - |
substrate phosphotyrosine | Burkholderia cenocepacia |
| 14.72 | - |
substrate p-nitrophenyl phosphate | Burkholderia cenocepacia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-phosphoserine + H2O | - |
Burkholderia cenocepacia | serine + phosphate | - |
? | |
| O-phosphotyrosine + H2O | - |
Burkholderia cenocepacia | tyrosine + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | activities towards tyrosine phosphatase substrate ENCpYINASL and calcineurin substrate DLDVPIPGRDFRRVpSVAAE were also detected, further substrates: ATP, sn-glycerol 3-phosphate | Burkholderia cenocepacia | p-nitrophenol + phosphate | - |
? | |
| phosphoenolpyruvate + H2O | - |
Burkholderia cenocepacia | pyruvate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 57000, SDS-PAGE, gel filtration | Burkholderia cenocepacia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| atypical purple alkaline phosphatase | - |
Burkholderia cenocepacia |
| purple acid phosphatase-like protein | - |
Burkholderia cenocepacia |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14.1 | - |
phosphoenolpyruvate | - |
Burkholderia cenocepacia | |
| 48.3 | - |
O-phosphoserine | - |
Burkholderia cenocepacia | |
| 138.1 | - |
O-Phosphotyrosine | - |
Burkholderia cenocepacia | |
| 147.9 | - |
p-nitrophenyl phosphate | - |
Burkholderia cenocepacia | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Burkholderia cenocepacia |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Burkholderia cenocepacia | induced by phosphate deficiency | up |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 40.8 | - |
O-phosphoserine | - |
Burkholderia cenocepacia | |
| 94.4 | - |
phosphoenolpyruvate | - |
Burkholderia cenocepacia | |
| 171 | - |
O-Phosphotyrosine | - |
Burkholderia cenocepacia | |
| 448 | - |
p-nitrophenyl phosphate | - |
Burkholderia cenocepacia | |
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Release 2023.1 (January 2023)
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