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Literature summary for 3.1.3.1 extracted from

  • Furuhashi, K.
    Alkaline phosphatase of Physarum polycephalum is insoluble (2008), Arch. Microbiol., 189, 151-156.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-glycerophosphate
-
Physarum polycephalum
NaF
-
Physarum polycephalum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requires Mg2+ Physarum polycephalum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
SDS-PAGE, non-reducing condition Physarum polycephalum
105000
-
SDS-PAGE, reducing condition Physarum polycephalum

Organism

Organism UniProt Comment Textmining
Physarum polycephalum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using column chromatography and preparative electrophoresis Physarum polycephalum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Physarum polycephalum 4-nitrophenol + phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 105000, SDS-PAGE under reducing conditions Physarum polycephalum

Synonyms

Synonyms Comment Organism
alkaline phosphatase
-
Physarum polycephalum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Physarum polycephalum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
Physarum polycephalum

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.8 no enzymatic activity below pH 6.2 or higher than pH 9.2 Physarum polycephalum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
18
-
-
Physarum polycephalum 2-glycerophosphate
49
-
-
Physarum polycephalum NaF