Literature summary for 3.1.3.1 extracted from

Nilgiriwala, K.S.; Alahari, A.; Rao, A.S.; Apte, S.K.
Cloning and overexpression of alkaline phosphatase PhoK from Sphingomonas sp. strain BSAR-1 for bioprecipitation of uranium from alkaline solutions (2008), Appl. Environ. Microbiol., 74, 5516-5523.

Application

Application	Comment	Organism
biotechnology	the data demonstrate the potential utility of genetically engineering PhoK for the bioprecipitation of uranium from alkaline solutions	Sphingomonas sp. BSAR-1

Cloned(Commentary)

Cloned (Comment)	Organism
phoK is cloned and overexpressed in Escherichia coli strain BL21(DE3). The resultant Escherichia coli strain EK4 overexpress cellular activity 55times higher and secrete extracellular PhoK activity 13times higher than Sphingomonas sp. strain BSAR-1. The recombinant strain very rapidly precipitate more that 90% of input uranium in less than 2 h from alkaline solutions (pH 9) containing 0.5 to 5 mM of uranyl carbonate. In recombinant strain EK4, precipitated uranium remains cell bound. The EK4 cells exhibited a much higher loading capacity of 3.8 g uranium/g dry weight in less than 2 h compared to only 1.5 g uranium/g dry weight in more than 7 h in strain BSAR-1	Sphingomonas sp. BSAR-1

Cloned (Comment)

Organism

phoK is cloned and overexpressed in Escherichia coli strain BL21(DE3). The resultant Escherichia coli strain EK4 overexpress cellular activity 55times higher and secrete extracellular PhoK activity 13times higher than Sphingomonas sp. strain BSAR-1. The recombinant strain very rapidly precipitate more that 90% of input uranium in less than 2 h from alkaline solutions (pH 9) containing 0.5 to 5 mM of uranyl carbonate. In recombinant strain EK4, precipitated uranium remains cell bound. The EK4 cells exhibited a much higher loading capacity of 3.8 g uranium/g dry weight in less than 2 h compared to only 1.5 g uranium/g dry weight in more than 7 h in strain BSAR-1

Sphingomonas sp. BSAR-1

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	x * 60000, SDS-PAGE	Sphingomonas sp. BSAR-1
200000	-	approximately, gel filtration	Sphingomonas sp. BSAR-1

Organism

Organism	UniProt	Comment	Textmining
Sphingomonas sp. BSAR-1	A1YYW7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl phosphate + H2O	-	Sphingomonas sp. BSAR-1	4-nitrophenol + phosphate	-	?
beta-glycerophosphate + H2O	uranium precipitation assay: uranyl carbonate solution in carbonate-bicarbonate buffer, supplemented with beta-glycerophosphate as the substrate at 30°C under static nongrowing conditions in a final volume of 5 ml	Sphingomonas sp. BSAR-1	glycerol + phosphate	-	?

Subunits

Subunits	Comment	Organism
multimer	x * 60000, SDS-PAGE	Sphingomonas sp. BSAR-1

Synonyms

Synonyms	Comment	Organism
alkaline phosphatase	-	Sphingomonas sp. BSAR-1
phoK	-	Sphingomonas sp. BSAR-1

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Sphingomonas sp. BSAR-1

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	assay at	Sphingomonas sp. BSAR-1