Cloned (Comment) | Organism |
---|---|
expressed under the control of the T7lac promoter on the expression vector pET-22b(+) in Escherichia coli BL21(DE3) | Thermus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Thermus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.039 | - |
4-nitrophenyl phosphate | 80°C, pH 12 | Thermus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, activates | Thermus sp. | |
Mg2+ | 1 mM, 10fold activation | Thermus sp. | |
Mn2+ | 1 mM, slight activation | Thermus sp. | |
Zn2+ | 1 mM, slight activation | Thermus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000, SDS-PAGE | Thermus sp. |
54407 | - |
x * 54407, calculated from sequence | Thermus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. | B8K1W1 | - |
- |
Thermus sp. T351 | B8K1W1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Thermus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30.8 | - |
- |
Thermus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyadenosine 5'-monophosphate + H2O | 113.3% of the activity with 4-nitrophenyl phosphate | Thermus sp. | ? | - |
? | |
2'-deoxyadenosine 5'-monophosphate + H2O | 113.3% of the activity with 4-nitrophenyl phosphate | Thermus sp. T351 | ? | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Thermus sp. | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Thermus sp. T351 | 4-nitrophenol + phosphate | - |
? | |
5-bromo-4-chloro-3-indolyl phosphate + H2O | 50.3% of the activity with 4-nitrophenyl phosphate | Thermus sp. | ? | - |
? | |
5-bromo-4-chloro-3-indolyl phosphate + H2O | 50.3% of the activity with 4-nitrophenyl phosphate | Thermus sp. T351 | ? | - |
? | |
adenosine 5'-monophosphate + H2O | 306.1% of the activity with 4-nitrophenyl phosphate | Thermus sp. | ? | - |
? | |
adenosine 5'-triphosphate + H2O | 257.4% of the activity with 4-nitrophenyl phosphate | Thermus sp. | ? | - |
? | |
alpha-naphthyl acid phosphate + H2O | 88.6% of the activity with 4-nitrophenyl phosphate | Thermus sp. | ? | - |
? | |
bis(p-nitrophenyl) phosphate + H2O | 6.1% of the activity with 4-nitrophenyl phosphate | Thermus sp. | ? | - |
? | |
bis(p-nitrophenyl) phosphate + H2O | 6.1% of the activity with 4-nitrophenyl phosphate | Thermus sp. T351 | ? | - |
? | |
cytidine 5'-monophosphate + H2O | 343.8% of the activity with 4-nitrophenyl phosphate | Thermus sp. | ? | - |
? | |
glycerophosphate + H2O | 248.7% of the activity with 4-nitrophenyl phosphate | Thermus sp. | ? | - |
? | |
glycerophosphate + H2O | 248.7% of the activity with 4-nitrophenyl phosphate | Thermus sp. T351 | ? | - |
? | |
guanosine 5'-monophosphate + H2O | 358.8% of the activity with 4-nitrophenyl phosphate | Thermus sp. | ? | - |
? | |
uridine 5'-monophosphate + H2O | 388.6% of the activity with 4-nitrophenyl phosphate | Thermus sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, SDS-PAGE | Thermus sp. |
? | x * 54407, calculated from sequence | Thermus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | 60°C: aout 50% of maximal activity, 90°C: about 40% of maximal activity | Thermus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
half-life is 103 min | Thermus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
12 | - |
- |
Thermus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 12 | 25°C, 16 h, stable | Thermus sp. |