Cloned (Comment) | Organism |
---|---|
circularly permutated alkaline phosphatase, expression in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | alkaline phosphatase is subject to circular permutation with its novel termini at the loops near the active site, and the original termini are linked by a flexible linker. While a permutant with the termini at original residues 407 and 408 is not active, a permutant with termini at residues 90 and 94 shows significant activity. Also the addition of a randomized residue at position 91 and 93 as well as outer peptide epitopes yields several mutants with specific activity comparable to the wild-type enzyme with similar outer peptides | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00634 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant circularly permutated alkaline phosphatase | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl phosphate + H2O | - |
Escherichia coli | p-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PHOA | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli |