Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme exhibits highest activity at 50 mM Gly-NaOH | Shewanella sp. |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Shewanella sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | 200 mM, 30% inhibition | Shewanella sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | enzyme exhibits highest activity in presence of 100 mM KCl | Shewanella sp. | |
Mg2+ | activity is greatly enhanced by simultaneous addition of Mg2+ and Zn2+. Maximal specific activity in presence of 20 mM MgCl2 and 5 mM ZnCl2 | Shewanella sp. | |
Zn2+ | activity is greatly enhanced by simultaneous addition of Mg2+ and Zn2+. Maximal specific activity in presence of 20 mM MgCl2 and 5 mM ZnCl2 | Shewanella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella sp. | - |
SIB1 | - |
Shewanella sp. SIB1 | - |
SIB1 | - |
Purification (Comment) | Organism |
---|---|
recombinant | Shewanella sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
950 | - |
- |
Shewanella sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl phosphate + H2O | - |
Shewanella sp. | p-nitrophenol + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Shewanella sp. SIB1 | p-nitrophenol + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Shewanella sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | 20°C: about 50% of maximal activity, 60°C: about 60% of maximal activity | Shewanella sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life: 22 min | Shewanella sp. |
70 | - |
half-life: 3.9 min | Shewanella sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.5 | - |
- |
Shewanella sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9.7 | 11.5 | pH 9.7: about 60% of maximal activity, pH 11.5: about 40% of maximal activity | Shewanella sp. |