Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.1 extracted from

  • Suzuki, Y.; Mizutani, Y.; Tsuji, T.; Ohtani, N.; Takano, K.; Haruki, M.; Morikawa, M.; Kanaya, S.
    Gene cloning, overproduction, and characterization of thermolabile alkaline phosphatase from a psychrotrophic bacterium (2005), Biosci. Biotechnol. Biochem., 69, 364-373.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme exhibits highest activity at 50 mM Gly-NaOH Shewanella sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Shewanella sp.

Inhibitors

Inhibitors Comment Organism Structure
NaCl 200 mM, 30% inhibition Shewanella sp.

Metals/Ions

Metals/Ions Comment Organism Structure
KCl enzyme exhibits highest activity in presence of 100 mM KCl Shewanella sp.
Mg2+ activity is greatly enhanced by simultaneous addition of Mg2+ and Zn2+. Maximal specific activity in presence of 20 mM MgCl2 and 5 mM ZnCl2 Shewanella sp.
Zn2+ activity is greatly enhanced by simultaneous addition of Mg2+ and Zn2+. Maximal specific activity in presence of 20 mM MgCl2 and 5 mM ZnCl2 Shewanella sp.

Organism

Organism UniProt Comment Textmining
Shewanella sp.
-
SIB1
-
Shewanella sp. SIB1
-
SIB1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Shewanella sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
950
-
-
Shewanella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl phosphate + H2O
-
Shewanella sp. p-nitrophenol + phosphate
-
?
p-nitrophenyl phosphate + H2O
-
Shewanella sp. SIB1 p-nitrophenol + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Shewanella sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 20°C: about 50% of maximal activity, 60°C: about 60% of maximal activity Shewanella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
half-life: 22 min Shewanella sp.
70
-
half-life: 3.9 min Shewanella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
Shewanella sp.

pH Range

pH Minimum pH Maximum Comment Organism
9.7 11.5 pH 9.7: about 60% of maximal activity, pH 11.5: about 40% of maximal activity Shewanella sp.