Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.1 extracted from

  • Ubeidat, M.; Rutherford, C.L.
    Purification and renaturation of Dictyostelium recombinant alkaline phosphatase by continuous elution electrophoresis (2003), Protein Expr. Purif., 27, 375-383.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
94% fragment of gene alp cDNA, functional overexpression in Escherichia coli BL21(DE3) as internally His-tagged enzyme use of continous electrophoresis procedure Dictyostelium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
x * 80000, recombinant enzyme, SDS-PAGE Dictyostelium sp.

Organism

Organism UniProt Comment Textmining
Dictyostelium sp.
-
gene alp
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme in nclusion bodies after expression in Escherichia coli Dictyostelium sp.

Reaction

Reaction Comment Organism Reaction ID
a phosphate monoester + H2O = an alcohol + phosphate Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate Dictyostelium sp.

Renatured (Commentary)

Renatured (Comment) Organism
recombinant His-tagged enzyme from inclusion bodies after expression in Escherichia coli, solubilization by 1% SDS in ddH2O from purified inclusion bodies Dictyostelium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.08
-
purified enzyme, substrate cAMP Dictyostelium sp.
0.33
-
purified enzyme, substrate ATP Dictyostelium sp.
0.65
-
purified enzyme, substrate beta-glycerol phosphate Dictyostelium sp.
0.7
-
purified enzyme, substrates 4-nitrophenyl phosphate and 5'-AMP Dictyostelium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Dictyostelium sp. 4-nitrophenol + phosphate
-
?
5'-AMP + H2O
-
Dictyostelium sp. adenosine + phosphate
-
?
ATP + H2O
-
Dictyostelium sp. ADP + phosphate
-
?
beta-glycerol phosphate + H2O
-
Dictyostelium sp. glycerol + phosphate
-
?
cAMP + H2O
-
Dictyostelium sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 80000, recombinant enzyme, SDS-PAGE Dictyostelium sp.

Synonyms

Synonyms Comment Organism
alkaline phosphatase
-
Dictyostelium sp.
ALP
-
Dictyostelium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Dictyostelium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
approximately Dictyostelium sp.