Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.1 extracted from

  • Hehir, M.J.; Murphy, J.E.; Kantrowitz, E.R.
    Characterization of heterodimeric alkaline phosphatases from Escherichia coli: An Investigation of Intragenic Complementation (2000), J. Mol. Biol., 304, 645-656.
    View publication on PubMed
Search for more literature for 3.1.3.1

Activating Compound

Activating Compound Comment Organism Structure
Mg2+
-
 Escherichia coli
Zn2+ stimulation of mutant enzymes, in presence or absence of a phosphoryl group acceptor in the reaction, except for mutnat H412Y, whose activity is reduced in presence of phosphoryl group acceptor and 0.1 mM Zn2+ Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutants in several Escherichia coli strains, intragenic complementation Escherichia coli

Protein Variants

Protein Variants Comment Organism
D369N site-directed mutagenesis, highly reduced activity Escherichia coli
D434E site-directed mutagenesis, reduced activity, increased kcat and Km compared to the wild-type enzyme Escherichia coli
E322K site-directed mutagenesis, highly reduced activity Escherichia coli
E341K site-directed mutagenesis, highly reduced activity Escherichia coli
H412Y site-directed mutagenesis, highly reduced activity Escherichia coli
S105L site-directed mutagenesis, reduced activity Escherichia coli
T155M site-directed mutagenesis, highly reduced activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ mutant H412Y in presence of a phosphoryl group acceptor and Zn2+ at 0.1 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic characterization of the homodimeric complementation mutants, and heterodimeric hybrid formation Escherichia coli
0.0035
-
 4-nitrophenyl phosphate mutant S105L, pH 8.0, 25°C, in absence of a phosphoryl group acceptor Escherichia coli
0.005
-
 4-nitrophenyl phosphate mutant T155M, pH 8.0, 25°C, in absence of a phosphoryl group acceptor Escherichia coli
0.0073
-
 4-nitrophenyl phosphate mutant H412Y, pH 8.0, 25°C, in absence of a phosphoryl group acceptor Escherichia coli
0.0094
-
 4-nitrophenyl phosphate wild-type enzyme, pH 8.0, 25°C, in absence of a phosphoryl group acceptor Escherichia coli
0.0095
-
 4-nitrophenyl phosphate mutant E341K, pH 8.0, 25°C, in absence of a phosphoryl group acceptor Escherichia coli
0.0097
-
 4-nitrophenyl phosphate mutant S105L, pH 8.0, 25°C, in presence of a phosphoryl group acceptor Escherichia coli
0.011
-
 4-nitrophenyl phosphate mutant E322K, pH 8.0, 25°C, in presence of a phosphoryl group acceptor Escherichia coli
0.0176
-
 4-nitrophenyl phosphate mutant D369N, pH 8.0, 25°C, in absence of a phosphoryl group acceptor Escherichia coli
0.0211
-
 4-nitrophenyl phosphate wild-type enzyme, pH 8.0, 25°C, in presence of a phosphoryl group acceptor Escherichia coli
0.0221
-
 4-nitrophenyl phosphate mutant E322K, pH 8.0, 25°C, in absence of a phosphoryl group acceptor Escherichia coli
0.0374
-
 4-nitrophenyl phosphate mutant E341K, pH 8.0, 25°C, in presence of a phosphoryl group acceptor Escherichia coli
0.057
-
 4-nitrophenyl phosphate mutant T155M, pH 8.0, 25°C, in presence of a phosphoryl group acceptor Escherichia coli
0.0597
-
 4-nitrophenyl phosphate mutant D369N, pH 8.0, 25°C, in presence of a phosphoryl group acceptor Escherichia coli
0.0691
-
 4-nitrophenyl phosphate mutant D434E, pH 8.0, 25°C, in absence of a phosphoryl group acceptor Escherichia coli
0.198
-
 4-nitrophenyl phosphate mutant H412Y, pH 8.0, 25°C, in presence of a phosphoryl group acceptor Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene phoA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutants, isolation of heterodimeric forms Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
a phosphate monoester + H2O = an alcohol + phosphate Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Escherichia coli 4-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
additional information heterodimer formation in vitro, overview, structural assembly of the enzyme into dimer induces cooperative interactions between the monomers necessary for the formation of the functional form of the holoenzyme Escherichia coli

Synonyms

Synonyms Comment Organism
alkaline phosphatase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Escherichia coli
0.016
-
 4-nitrophenyl phosphate mutant E322K, pH 8.0, 25°C, in absence of a phosphoryl group acceptor Escherichia coli
0.027
-
 4-nitrophenyl phosphate mutant E322K, pH 8.0, 25°C, in presence of a phosphoryl group acceptor Escherichia coli
0.042
-
 4-nitrophenyl phosphate mutant E341K, pH 8.0, 25°C, in absence of a phosphoryl group acceptor Escherichia coli
0.052
-
 4-nitrophenyl phosphate mutant T155M, pH 8.0, 25°C, in absence of a phosphoryl group acceptor Escherichia coli
0.059
-
 4-nitrophenyl phosphate mutant H412Y, pH 8.0, 25°C, in presence of a phosphoryl group acceptor Escherichia coli
0.067
-
 4-nitrophenyl phosphate mutant H412Y, pH 8.0, 25°C, in absence of a phosphoryl group acceptor Escherichia coli
0.1
-
 4-nitrophenyl phosphate mutant E341K, pH 8.0, 25°C, in presence of a phosphoryl group acceptor Escherichia coli
0.32
-
 4-nitrophenyl phosphate mutant T155M, pH 8.0, 25°C, in presence of a phosphoryl group acceptor Escherichia coli
0.39
-
 4-nitrophenyl phosphate mutant D369N, pH 8.0, 25°C, in absence of a phosphoryl group acceptor Escherichia coli
2.4
-
 4-nitrophenyl phosphate mutant D369N, pH 8.0, 25°C, in presence of a phosphoryl group acceptor Escherichia coli
3.8
-
 4-nitrophenyl phosphate mutant S105L, pH 8.0, 25°C, in absence of a phosphoryl group acceptor Escherichia coli
5.8
-
 4-nitrophenyl phosphate mutant S105L, pH 8.0, 25°C, in presence of a phosphoryl group acceptor Escherichia coli
44.4
-
 4-nitrophenyl phosphate wild-type enzyme, pH 8.0, 25°C, in absence of a phosphoryl group acceptor Escherichia coli
65.6
-
 4-nitrophenyl phosphate mutant D434E, pH 8.0, 25°C, in absence of a phosphoryl group acceptor Escherichia coli
80.5
-
 4-nitrophenyl phosphate wild-type enzyme, pH 8.0, 25°C, in presence of a phosphoryl group acceptor Escherichia coli