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Literature summary for 3.1.3.1 extracted from

  • Wennberg, C.; Kozlenkov, A.; Di Mauro, S.; Frohlander, N.; Beckman, L.; Hoylaerts, M.F.; Millan, J.L.
    Structure, genomic DNA typing, and kinetic characterization of the D allozyme of placental alkaline phosphatase (PLAP/ALPP) (2002), Hum. Mutat., 19, 258-267.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
allozyme D, ALPP, of placental enzyme form PALP, DNA and amino acid sequence determination of PALP and allozymes, comparison and analysis, substitutions from PALP to ALPP are found at P209R and E429G, subcloning in Escherichia coli XL1-blue, and expression of allozymes D, ALPP, in CHO cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
L-leucine allozyme D is highly sensitive and shows uncompetitive inhibition, allozymes S and F are less sensitive, allozymes SD and FD, mixed type, respond in an intermediate fashion to inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of other allozymes of PALP Homo sapiens
0.11
-
4-nitrophenyl phosphate allozyme D, pH 9.8, in presence of Mg2+ and Zn2+ Homo sapiens
0.12
-
pyridoxal 5'-phosphate allozyme D, pH 7.5, 37°C, in presence of Mg2+ and Zn2+ Homo sapiens
1.4
-
diphosphate allozyme D, pH 7.5, 37°C, in presence of Mg2+ and Zn2+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Homo sapiens
-
2 phosphate
-
?
pyridoxal 5'-phosphate + H2O Homo sapiens
-
pyridoxal + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
a phosphate monoester + H2O = an alcohol + phosphate Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta several allozymes of placental enzyme form PALP Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Homo sapiens 4-nitrophenol + phosphate
-
?
diphosphate + H2O
-
Homo sapiens 2 phosphate
-
?
pyridoxal 5'-phosphate + H2O
-
Homo sapiens pyridoxal + phosphate
-
?

Synonyms

Synonyms Comment Organism
ALPP
-
Homo sapiens
AP
-
Homo sapiens
PALP
-
Homo sapiens
placental alkaline phosphatase
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
61
-
allozyme D, half-life 10 min, in presence of Zn2+ and Mg2+, pH 9.8 Homo sapiens
64
-
allozyme SD and FD, half-life 10 min, in presence of Zn2+ and Mg2+, pH 9.8 Homo sapiens
72
-
allozyme S and F, half-life 10 min, in presence of Zn2+ and Mg2+, pH 9.8 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Homo sapiens
92
-
pyridoxal 5'-phosphate allozyme D, pH 7.5, 37°C, in presence of Mg2+ and Zn2+ Homo sapiens
244
-
diphosphate allozyme D, pH 7.5, 37°C, in presence of Mg2+ and Zn2+ Homo sapiens
379
-
4-nitrophenyl phosphate allozyme D, pH 9.8, in presence of Mg2+ and Zn2+ Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay with physiological substrates Homo sapiens
9.8
-
optimum, assay with 4-nitrophenyl phosphate Homo sapiens