Cloned (Comment) | Organism |
---|---|
allozyme D, ALPP, of placental enzyme form PALP, DNA and amino acid sequence determination of PALP and allozymes, comparison and analysis, substitutions from PALP to ALPP are found at P209R and E429G, subcloning in Escherichia coli XL1-blue, and expression of allozymes D, ALPP, in CHO cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-leucine | allozyme D is highly sensitive and shows uncompetitive inhibition, allozymes S and F are less sensitive, allozymes SD and FD, mixed type, respond in an intermediate fashion to inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of other allozymes of PALP | Homo sapiens | |
0.11 | - |
4-nitrophenyl phosphate | allozyme D, pH 9.8, in presence of Mg2+ and Zn2+ | Homo sapiens | |
0.12 | - |
pyridoxal 5'-phosphate | allozyme D, pH 7.5, 37°C, in presence of Mg2+ and Zn2+ | Homo sapiens | |
1.4 | - |
diphosphate | allozyme D, pH 7.5, 37°C, in presence of Mg2+ and Zn2+ | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Homo sapiens | - |
2 phosphate | - |
? | |
pyridoxal 5'-phosphate + H2O | Homo sapiens | - |
pyridoxal + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a phosphate monoester + H2O = an alcohol + phosphate | Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | several allozymes of placental enzyme form PALP | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? | |
diphosphate + H2O | - |
Homo sapiens | 2 phosphate | - |
? | |
pyridoxal 5'-phosphate + H2O | - |
Homo sapiens | pyridoxal + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALPP | - |
Homo sapiens |
AP | - |
Homo sapiens |
PALP | - |
Homo sapiens |
placental alkaline phosphatase | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
61 | - |
allozyme D, half-life 10 min, in presence of Zn2+ and Mg2+, pH 9.8 | Homo sapiens |
64 | - |
allozyme SD and FD, half-life 10 min, in presence of Zn2+ and Mg2+, pH 9.8 | Homo sapiens |
72 | - |
allozyme S and F, half-life 10 min, in presence of Zn2+ and Mg2+, pH 9.8 | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens | |
92 | - |
pyridoxal 5'-phosphate | allozyme D, pH 7.5, 37°C, in presence of Mg2+ and Zn2+ | Homo sapiens | |
244 | - |
diphosphate | allozyme D, pH 7.5, 37°C, in presence of Mg2+ and Zn2+ | Homo sapiens | |
379 | - |
4-nitrophenyl phosphate | allozyme D, pH 9.8, in presence of Mg2+ and Zn2+ | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay with physiological substrates | Homo sapiens |
9.8 | - |
optimum, assay with 4-nitrophenyl phosphate | Homo sapiens |