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Literature summary for 3.1.3.1 extracted from

  • Guimaraes, L.H.; Terenzi, H.F.; Jorge, J.A.; Leone, F.A.; Polizeli, M.L.
    Extracellular alkaline phosphatase from the filamentous fungus Aspergillus caespitosus: purification and biochemical characterization (2003), FEMS Microbiol. Rev., 48, 627-632.
    View publication on PubMed
Search for more literature for 3.1.3.1

Activating Compound

Activating Compound Comment Organism Structure
Mg2+ 95% activation at 1 mM Aspergillus caespitosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.074
-
 4-nitrophenyl phosphate in absence of Mg2+ Aspergillus caespitosus
0.09
-
 4-nitrophenyl phosphate in presence of Mg2+ Aspergillus caespitosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus caespitosus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 2 * 57000, SDS-PAGE Aspergillus caespitosus
134800
-
 gel filtration Aspergillus caespitosus

Organism

Organism UniProt Comment Textmining
Aspergillus caespitosus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzyme contains up to 56% carbohydrate Aspergillus caespitosus

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus caespitosus

Reaction

Reaction Comment Organism Reaction ID
a phosphate monoester + H2O = an alcohol + phosphate Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate Aspergillus caespitosus
a

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:xylan-grown cell
-
 Aspergillus caespitosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Aspergillus caespitosus 4-nitrophenol + phosphate
-
 ?
O-phosphoserine + H2O
-
 Aspergillus caespitosus serine + phosphate
-
 ?
O-phosphothreonine + H2O
-
 Aspergillus caespitosus threonine + phosphate
-
 ?
O-phosphotyrosine + H2O
-
 Aspergillus caespitosus tyrosine + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 57000, SDS-PAGE Aspergillus caespitosus

Synonyms

Synonyms Comment Organism
alkaline phosphatase
-
 Aspergillus caespitosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Aspergillus caespitosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable at Aspergillus caespitosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Aspergillus caespitosus